Structure and Function of Ocular Ciliary Epithelium

采用多种电镜技术观察纤毛上皮的精细结构。兔眼睫状体的虹膜部分被认为是分泌房水最活跃的部位。上皮由着色（PE）和非着色（NPE）上皮层组成。在冻裂标本中，PE和NPE上皮细胞的基底外侧细胞表面都显示出与相邻上皮细胞交错的复杂的褶皱，它们的细胞膜含有丰富的膜内颗粒。发现上皮内神经纤维。呈串珠状分布于PE上皮细胞内。它们的扩张部分含有两种囊泡。5′-羟多巴胺处理后的小囊泡（直径-50 nm）含有致密颗粒，表明其具有去甲肾上腺素能性质。局部应用福斯可林引起以下形态学变化；睫状体突间质水肿，睫状体通道及PE细胞间隙扩张。结果表明，该剂引起了水的再吸收。采用胶原酶溶液灌注法成功分离完整纤毛上皮双分子层（PE+NPE）。研究了Na/K atp酶在新鲜分离双分子层标本上的免疫细胞化学定位。电镜观察显示，酶定位于细胞膜沿基底侧表面的PE和NPE。研究结果表明，这两层细胞都参与房水的产生。利用这种分离的上皮细胞薄片进行的生理和药理学实验正在进行中，并且已经获得了令人鼓舞的结果。这些实验是与耶鲁大学医学院的M. Sears教授合作进行的。

The fine structure of ciliary epithelium was investigated by various techniques of electron microscopy. The iridial portion of ciliary process in rabbit eye was mainly used, where the aqueous humor production is supposed to be most active.1. The epithelium is consisted of pigmented (PE) and nonpigmented (NPE) epithelial layer. The basolateral cell surfaces of both PE and NPE epitheliocyte show elaborate infoldings which interdigitate with those of adjacent epitheliocytes, and their cell membrane contains abundant intramembrane particles in the freeze fractured specimen.2. The intraepithelial nerve fibers were found. They showed beaded appearance and distributed among PE epitheliocytes. They contain 2 kinds of vesicle in their dilated portion. The smaller vesicles (-50 nm in diameter) contain dense granule after treatment with 5' -hydroxydopamine, thus indicated thus indicated their noradrenergic nature.3. The topical application of forskolin induced following morphological changes ; oedemous stroma of ciliary process, and dilation of ciliary channel as well as intercellular space of PE. The findings suggest that the agent caused the reabsorption of the aqueous.4. The intact ciliary epithelial bilayer (PE+NPE) was successfully isolated by perfusion method using collagenase solution. The immunocytochemical localization of Na/K ATPase was investigated on the isolated fresh bilayer specimen. The EM observation revealed that the enzyme localizes on the cell membrane along the basolateral surfaces of both PE and NPE. The findings suggest both cell layers participate in aqueous humor production.5. The physiological and pharmacological experiment is underway using this isolated epithelial sheet and promising results are already obtained.These experiments were collaborated with Prof. M. Sears, Yale University School of Medicine.