Research of the Death from Drug Induced Anaphylactic Shock

本研究的目的是为了从死后材料中证明药物引起的过敏反应。研究结果如下：1。给药化合物48/80是一种肥大细胞脱颗粒剂，在法医学领域是一种方便而有用的方法，用于建立过敏反应死亡的实验动物模型，而不是使用通常的免疫方法。全血必须作为死后的血液样本。这是因为血浆和血清由于凝血、溶血和死后变化引起的变性而不能明显分离。3 .犬死后血液中最重要的休克介质组胺（HA）和HA的代谢物1-甲基组胺（MHA）含量的测定，需要用阳离子交换纤维素进行脱蛋白、提取和纯化，并采用无干扰物荧光检测器的高效液相色谱法。死后24小时血液中的血凝素水平急剧上升，即使在死前血凝素没有释放到血液中。只有在死前大量HA存在时，MHA水平才会在死后显著升高。体外免疫球蛋白E （IgE）抗体反应在20c下稳定3 d。即使在无法从尸体中获得血液的情况下，也可以使用内脏匀浆的上清在死亡后长达3天的时间内测量半抗原特异性IgE抗体反应。然而，这项研究并不直接适用于人类，因为死亡前后的情况和条件，例如疾病和紧急情况等，是完全不同的。然而，这些发现，特别是关于实验动物模型和IgE抗体的稳定性，表明能够证明过敏性死亡的可能性。

This study was undertaken for the purposes of the certifying drug-induced anaphylaxis from postmortem materials. The findings were as follows :1. Administrating Compound 48/80, a degranulating agent of mast cells, is a convenient and useful method in the field of forensic medicine, for creating an experimental animal model of death from anaphylaxis instead of using the usual immunological method.2. Whole blood must be used as the postmortem sample for blood. This is because neither plasma nor serum can be clearly separated because of clotting, hemolysis and degeneration due to postmortem changes.3. Deproteinization, extraction and purification with cation-exchange cellulose were needed for the determination of Histamine (HA), one of the most important shock mediators, and 1-Methylhistamine (MHA), one of the metabolites of HA, levels in dog's blood after death, using high-performance liquid chromatography with a fluorometric detector without interfering substances.4. HA levels in blood were tremendously increased about 24 hours after death, even without a release of HA into the blood just before death. MHA levels were considerably increased after death, only when a lot of HA had been present just before death.5. In vitro immunoglobulin E (IgE) antibody responses in blood were stable for 3 days at 20ﾟC. Even in cases when blood could not be obtained from the cadaver, it was possible to measure the hapten specific IgE antibody responses for up to 3 days after death using the supernatant of the visceral homogenates.This study, however, is not directly applicable to humans, because the situations and conditions both before and after death concerning, for example, disease and emergency etc, were completely different. However, these findings, especially concerning the experimental animal model and the stability of IgE antibody, suggest the possibility of being able to certify death from anaphylaxis.