Bioscientific study of the therapy for lung small cell cancer

1. 抗癌药物敏感性(1)手术标本癌细胞的制备：将肺癌组织切碎，用网片过滤，用Ficoll Paque分离。该方法存在的问题是：(1)残留在网片上的癌细胞数量不容忽视；(2)分离时淋巴细胞和成纤维细胞受到污染。酶（cllagenase, Dnase, trypsin）法存在如下问题：(1)酶处理后黏度增加，过滤困难；(2)酶处理后癌细胞生长速度下降。(2)培养基：添加10% FCS， RPM11640、HA M、IMDM和MEM之间细胞生长速率无差异。在这些培养基中，成纤维细胞比癌细胞增殖更明显。而在无血清培养基中，HITES（SCLC）、SCL-4（腺细胞）的成纤维细胞增殖受到抑制，癌细胞的存活时间比有血清的短。在2 - 1层以上琼脂糖培养基中，控制成纤维细胞增殖，检查癌细胞不增殖。这表明，在这些介质下，抗癌药物的敏感性试验不能超过一周的时间，而在72小时内的实验系统或癌细胞活性及其分子水平的另一种评价方法被认为是研究。肺癌宿主对淋巴细胞上Tac、KOLT-2、YTA-1抗原表达的免疫反应，但手术的影响尚不清楚，部分病例转移（+）淋巴细胞上KOLT-2抗原表达较转移（-）淋巴细胞增加。对IL-2或YTA-1的增殖反应有待研究。在许多恶性病例中，随着病变的切除，leu7的表达增加，而在良性病例中，手术后表达无明显变化。观察其与手术根治性、两色FACS与leull、NK活性等的关系。少

1. Sensitivity for anticancer drug (1)preparation of cancer cell from surgical specimen: Minced lung cancer tissue was filtrated though mesh, and separated by Ficoll Paque. The problems of this method were (1) the number of cancer cells whitc h remained on the mesh is not negligible, (2) at the step of separation, the contamination of lymphocyte s and fibroblast cells occurred. The enzyme(cllagenase, Dnase, trypsin) method had the Problems such as (1) viscosity was increased after enzyme treatment, making filtration rather difficult, 0 the growth rate of cancer cells decreased after this treatment.(2)culture medium: With 10% FCS, between RPM11640, HA M, IMDM, and MEM, there is no difference about cell growth rate. In these mediums, fibroblast cells proliferated significantly rather than cancer cells. With serum-free medium, HITES(SCLC), SCL-4(adeno), however, the proliferation of fibloblast cell was suppressed, survival period of cancer cell was shorter than that with serum. With two-l … More ayer agarose medium, fibloblast cells were controlled to proliferate, and examined cancer cells were not proliferated. This suggest that with these medium, sensitivity test of anti-cancer drugs can not be done over one week period, and experimental system within 72 hours or the another evaluation method of the activity of cancer cell and its molecular level are thought to be studied.2. Immunological response of the host with lung cancer To the expression of Tac, KOLT-2, YTA-1 antigens on lymphocyte, however, the effect of operation was not recognized,the expression of KOLT-2 antigen was augmented on the lymphocytes of metastasis(+) lymphonode in comparison with that of metastasis(-) lymphonode in some cases. The prolifalative response to IL-2 or YTA-1 is to be studied. In many malignant cases, the expression of leu7 was augmented with resection of its lesion, there was no remarkable change on operation in benign cases. The relation with operative radicality, two color FACS with leull, NK activity, and so on are to be examined. Less