Study on Regeneration of Periodontal Ligament around Dental Implants by Cell-seeding of Cultured Periodontal Ligament Cells

本研究的目的是通过体外培养的牙周膜细胞，通过实验确定牙周膜细胞的种植能否促进种植体周围新牙周膜的再生.为确定牙周膜细胞（PLC）的体外培养方法，进行了体外研究。结果表明，10%胎牛血清最适合PLC的生长和分化.胶原在牙周组织再生中起着重要作用，我们尝试将胶原固定在羟基磷灰石（HAP）、钛（Ti）和聚乙烯（PE）制成的牙种植体周围。结果表明，等离子体固化法可将胶原固定在PE种植体表面。而在HAP和Ti种植体上则不能.将PLC预培养，然后将HAP和胶原包被的PE植入物浸入培养基中并孵育以通过PLC附着。结果，HAP植入物在浸泡7天后被PLC完全覆盖，而胶原蛋白涂层的PE植入物没有，因为PE植入物太轻以至于漂浮在培养基上。将体外培养的PCL覆盖的HAP种植体植入到下颌牙槽突的无牙前磨牙区域钻的孔中，并完全由牙龈瓣覆盖。结果，植入3个月后，没有种植体感染，但种植体周围的放射线层在植入后早期逐渐闭合，没有牙周膜再生的直接证据。

The purpose of the present study was to experimentally determine if regeneration of new periodontal ligament around dental implants can be accomplished by cell-seeding of in-vitro cultured periodontal ligament cells.1. In-vitro investigations were made in order to determine the suitable culture method for periodontal ligament cells (PLCs). In result, culture with 10% FBS was considered to be most suitable for growth and differenciation of PLCs.2. Collagen which plays an important role in regeneration of periodontal tissues was tried to be immobilized around dental implants made of hydroxyapatite (HAP), titanium (Ti) and polyethylene (PE). In result, collagen could be immobilized onto the surface of PE implants by plasma curing method. but could not onto HAP and Ti implants.3. PLCs were pre-cultured and then HAP and collagen-coated PE implants were immersed into the medium and incubated to be attached by PLCs. In result, HAP implants were covered throughout by PLCs 7 days after immersed, whereas collagen-coated PE implants were not, because PE implants were so light as to float on the medium.4. HAP implants covered by in-vitro cultured PCLs were implanted to holes drilled in the edentulous premolar region of the mandibular alveolar process and completely covered by a gingival flap. In result, 3 months after implantation no implant became infected, but the radiolucent layers which had been seen around the implants early after implantation became gradually closed and no direct evidence of regenaration of periodontal ligament was given.