Involvement of ets oncogene family in chromosomal translocation and hemopoietic cell dofferentiation into granulocyte/macrophage lineage

1.造血细胞分化与ets癌基因探讨ets癌基因家族与造血细胞分化的关系。在北方印迹分析中，诱导分化为巨噬细胞谱系增强了ets癌基因家族之一的PU. 1的表达。ets家族的其他成员。然而，没有显示出显著变化。用PCR方法检测了几例白血病患者的白血病细胞中PU.1的表达，发现PU.1在单核细胞分化的白血病细胞中表达显著。提示PU.1信息的检测对白血病的临床分型有一定的意义.细胞功能与ets癌基因ets癌基因是转录因子，它们的识别序列见于各种淋巴因子启动子。ets识别序列的突变使这些启动子的转录活性消失。此外，ets与其他因子（加塔的NF-κ B）协同和协同地激活转录。这些结果表明ets家族在细胞功能中起重要作用.易位转录因子基因与肿瘤发生。我们正在研究白血病发生过程中一些转录因子重排的作用。

1. Hemopoietic cell differentiation and ets oncogenes Relationship between ets oncogene family and hemopoietic cell differentiation was investigated. In Northern blottong analysis, expression of PU.1, one of the ets oncogene family, was enhanced by the induction of differentiation to macrophage lineage. Other members of ets family. however, did not show remarkable change. Using leukemic cells from several leukemia patients, the expression of PU.1 was detected by PCR.The expression was remarkable in leukemic cells with monocytic differentiation. These observations suggested that te detection of PU.1 message may be useful in clinical typing of leukemia.2. Cell function and ets oncogenes ets oncogenes are transcription factors, and their recognition sequence are seen in various lymphokine promoters. Mutation of ets recognition sequence abolished the transcriptional activity of those promoters. In addition, ets activated transcription cooperatively and synergistically with other factors (NF-kB of GATA). These results indicated that ets family play an important role in cell functions.3. Translocated transcription factor gene and tumorigenesis. We are now investigating the role of some transcription factors which are rearranged during leukemogenesis.