Architecture and function of the organelle contact sites of the vacuole (P24)

膜接触位点是结合蛋白产生两个细胞器膜紧密附着而不导致融合的区域。这些结构是细胞器之间脂质和可溶性代谢物交换的平台，从而支持细胞内代谢的协调。我们最近发现了一种未知的蛋白质Cvm1，它是液泡、线粒体和过氧化物酶体之间接触位点的新成分。该项目将解决两个密切相关的问题。在第一部分中，将澄清Cvm1如何在液泡中创建不同的接触位点，它与不同细胞器上的哪些蛋白质相互作用，以及这些相互作用需要蛋白质内的哪些结构域。第二个目标侧重于Cvm1的功能及其形成的接触位点及其与细胞生理学的相关性。因此，该项目将在SFB中引入一种新的带有膜接触位点的微室，并与其他小组密切合作，阐明它们在细胞环境中的相关性。

Membrane contact sites are areas in which binding proteins produce a close attachment of the membranes of two organelles without leading to fusion. These structures are platforms that enable the exchange of lipids and soluble metabolites between organelles and thus support the coordination of metabolism in the cell. We have recently identified the uncharacterized protein Cvm1 as a new component of contact sites between vacuole, mitochondria and peroxisomes. The project will address two closely related issues. In the first part, it will be clarified how Cvm1 creates different contact sites at vacuoles, which proteins it interacts with on the different organelles and which domains within the protein are required for these interactions. The second goal focuses on the function of Cvm1 and the contact sites it forms and their relevance for cellular physiology. The project will thus introduce a new microcompartment into the SFB with membrane contact sites and clarify their relevance in the cellular context in close cooperation with other groups.