Linkage analysis for biochemical marker loci in quail

对日本鸣禽的血清白蛋白(ALB)、维生素D结合蛋白(GC)、前白蛋白(Pre-ALB)、蛋白酶抑制物(PI)、葡萄糖磷酸异构酶(GPI)、磷酸葡萄糖脱氢酶(PGD)和酯酶-D(Es-D)基因进行了遗传连锁分析。Alb、Pre-Alb、GPI、PGD和Es-D的多态已经在以前的文章中得到了证明。在本研究中，进一步通过等电聚焦(IEF)和免疫印迹的方法观察到GC基因的多态性。这些性状由两个常染色体共显性等位基因GC^A和GC^B控制。对7个座位间的双回交进行连锁分析。分离数据建立了Alb和Gc基因座之间的紧密连锁。重组频率估计为0.014&lt；正负0.006(P&lt；0.001)。Alb-GC复合体、前白蛋白、PI、GPI、PGD和Es-D LOC之间没有明显的连锁迹象

Serun albumin (ALB), vitamin D binding protein (GC), pre-albumin (Pre-ALB), protease inhibitor (PI), glucosephosphate isomerase (GPI), phosphogluconate dehydrogenase (PGD) and esterase-D (Es-D) polymorphisms in Japanese quail were investigated for genetic linkage analysis. ALB,Pre-ALB,GPI,PGD and Es-D polymorphisms were already demonstrared in previous articles. In this study, further GC polymorphism were observed by isoelectric focusing (IEF) and immunoblotting using rabbit antichicken GC.Three phenotypes were observed. These were controlled by two autosomal codominant alleles named Gc^A and Gc^B.Double backcrosses among seven loci were examined for the linkage analysis. The segregation data established close linkage between the Alb and Gc loci. The recombination frequency was estimated 0.014 <plus-minus> 0.006 (P<0.001). No clear signs of linkage were observed among Alb-Gc complex, Pre-alb, Pi, Gpi, Pgd and Es-D loc