RAPID DETECTION OF MYCOPLASMAS BY A FILTER-IMMUNOBINDING TECHNIQUE

建立了一种利用商业斑点板和强化硝酸纤维素膜硝基Plus 2000的过滤免疫结合（FIB）技术。当稀释的支原体液体培养物用作抗原时，用培养基吸附抗血清是至关重要的，以避免显著的非特异性FIB反应。吸附的抗血清与牛和禽支原体型菌株以及经生长抑制试验鉴定的田间分离株产生特异性FIB反应。FIB法对小牛鼻分泌物中牛鼻分支杆菌和牛分支杆菌的检出率略低于常规培养法。FIB法对鸡败血支原体和滑液支原体的检出率明显低于培养法，可能与正常鸡口咽部存在少量支原体有关。结果表明，FIB技术可用于支原体病的快速诊断。

A filter-immunobinding (FIB) technique in which commercial dot-plates and enforced nitrocellulose membrane, Nitro Plus 2000, were utilized, was developed. When diluted mycoplasma liquid cultures were used as antigens, it was critical to adsorb antisera with culture medium in order to avoid significant unspecific FIB reactions. Adsorbed antisera gave rise to specific FIB reactions with bovine and avian mycoplasma Type strains as well as field isolates identified by growth inhibition test. Rate of detection of M.bovirhinis and M.bovis from nasal discharge of calves by FIB was siightly less than by the routine culture method. Detection rate of M.gallisepticum and M.synoviae by FIB much less than that obtained by culture method, probablly due to small CFU of mycoplasmas existed in the oropharynxes of normal chickens examined. It was concluded that FIB technique was useful for the rapid diagnosis of mycoplasmoses in which great number of organisms might exist.