Serum Propeptide and Intact Molecular Osteocalcin in Normal Children and Children with Growth Hormone(GH) Deficiency

为了建立一个敏感的骨形成标记物，我们已经开发了一个完整的骨钙素（OC）及其前肽的夹心酶联免疫吸附试验。对185名4-15岁正常儿童和23名GH缺乏儿童的血清中上述肽水平进行了研究，正常青春期前儿童的血清中前肽水平为1.43 ± 0.05SY。（）0.23（平均值）SY. ±.男（）SE）μ g/L，女（）SY ± 1.53。（）女生0.23 μ g/L。男生13岁时达高峰（2.9 ± 0.001），男生13岁时达高峰（2.9 ± 0.001）。11岁组为2.34 ± 0.42 μ g/L。（）0.34 μ g/L。青春期前男女儿童血清完整OC水平分别为18.8 ± 0.00SY。（）2.1和20.7（）SY. ±.（）2.1 μ g/L，升高至41.0 ± 0.05 μ g/L。（）13岁~ 27.0岁男孩3.7mug/L（）SY. ±.（）11岁女孩2.5mug/L。在GH缺乏的患者中，GH治疗1个月后观察到前肽水平增加2.3倍，完整OC水平增加1.7倍。GH治疗1个月后的血清前肽和完整OC水平与GH治疗12个月后的生长反应相关（前肽r=0.660和P<0.01;完整OC r=0.537和P<0.01）。

To establish a sensitive marker for bone formation we have developed a sandwich enzyme-linked immunosorbent assay for intact osteocalcin(OC) and its propeptide. Serum levels of these peptides were studied in 185 normal children, aged 4-15 yr, and in 23 GH-deficient children treated with GH.The serum levels of the propeptide in normal prepubescent children were 1.43 (〕SY.+-.〔) 0.23 (mean (〕SY.+-.〔) SE)mug/L in boys and 1.53 (〕SY.+-.〔) 0.23mug/L in girls. The peak value occurred at the age of 13 yr in boys (2.9 (〕SY.+-.〔) 0.42mug/L) and 11 yr in girls (2.34 (〕SY.+-.〔) 0.34mug/L). The serum intact OC levels in prepubescent boys and girls were 18.8 (〕SY.+-.〔) 2.1 and 20.7 (〕SY.+-.〔) 2.1mug/L, respectively, and these levels increased to 41.0 (〕SY.+-.〔) 3.7mug/L in boys aged 13 yr and to 27.0 (〕SY.+-.〔) 2.5mug/L in girls aged 11 yr. In the GH-deficient patients, a 2.3-fold increase in the propeptide levels and a 1.7-fold increase in the intact OC level was observed after 1 month of GH therapy. Serum propeptide and intact OC levels after 1 month of GH therapy correlated with the growth response after 12 months of GH therapy(r=0.660 and P<0.01, for propeptide ; r=0.537 and P<0.01 for intact OC).