Pathogenic role of heat shock proteins gammadelta-T cells in inflammatory skin diseases

慢性和潜伏性感染常引起或触发身体远端(S)的致病过程，可用“局灶性感染”来解释。热休克蛋白(HSPs)是一种免疫保守性很好的蛋白质，在高温或其他环境中暴露后，在细胞中被诱导。由于细菌热休克蛋白是免疫原性的，是免疫监视的靶标，而感染应激后诱导的哺乳动物热休克蛋白能够被相同的免疫机制识别，因此这种现象与宿主对发育期的防御和自身免疫的诱导有关。我们开始研究分枝杆菌HSP65抗体在各种炎症性皮肤病中的产生，以确定这种所谓的“局灶性感染”。1992年，我们获得了大约200例各种皮肤病患者的数据。与正常组相比，掌跖脓疱病、银屑病、荨麻疹和带状疱疹患者的Ig…经常升高。酶联免疫吸附试验检测麻风分枝杆菌HSP65 G抗体。1993年，我们进一步研究了抗HSP65抗体与细菌感染所致皮肤炎症性疾病的相关性。新收集的PPP患者分为以慢性扁桃体炎或牙周炎为感染源和非慢性扁桃体炎或牙周炎为感染源的两组。在492 nm处的OD值在有感染灶的PPP(n=7)、0.230(Sy.+-)0.065、平均Sy(+-)SD(n=12、0.139(Sy+-)0.066)之间有显著差异(p&lt0.01)。在银屑病中，收集的银屑病患者根据皮损类型分为三组：急性点滴型银屑病(PGA)，这是一种特殊类型的银屑病，常由链球菌感染后诱发，斑块型银屑病(PV)和扁桃型银屑病(PP)。PGA(n=4)、PV(n=12)和PP(n=6)的抗HSP65Ig G水平分别为0.174、0.101、0.053和0.087、0.025，差异有统计学意义(p=0.0165，PGA-PV；p=0.001，PGA-PP)。抗HSP65Ig G升高与细菌感染引起的银屑病和皮肤白质炎症有关。较少

Chronic and latent infectious focus often causes or triggers the pathogenic process in distant part(s) of the body, being explained by a term "focal infection". Immunogenetically well-conserved proteins, heat shock proteins (HSPs), are inducied in cells after exposure to elevated temperature or others. This phenomenon has been investigated in relation to both host-defense to dacteria and induction of autoimmunity, since bacterial HSPs are immunogenic and targetted by immune surveilance, while mammalian HSPs induced after infectious stress are able to be recognized by the same immune mechanism. We started to investigate antibody production to mycobacterial HSP65 in various inflammatory skin diseases in order to correlate this so-called 'focal infection".In 1992, we obtained data from approximately 200 patients with various skin diseases. In comparison to normal group, patients with palmoplantar pustulosis (PPP), psoriasis (Pso), urticaria, and herpes zoster frequently showed elevated Ig … More G antibody to HSP65 from Mycobacterium leprae assayd on ELISA plates. In patients with anaphylactoid purpura the antibody level was not raised.In 1993, we further investigated correlation between antibody to HSP65 and skin inflammatory diseases triggered by bacterial infection. Patients with PPP were newly collected and divided into groups with and without chronic tonsilitis or periodontitis as infectious focus. OD at 492 nm in this ELISA technique revealed significant (p<0.01) difference between PPP with infectious focus (n=7,0.230(〕SY.+-.〔)0.065, mean(〕SY.+-.〔)SD) and PPP without it (n=12,0.139(〕SY.+-.〔)0.066). IN psoriasis, collected patients were divided into three groups according to types of psoriatic lesions : acute guttate psoriasis (PGA) that is a particular type of psoriasis induced often after steptococcal infection, plaque-type psoriasis (PV) and psutular-form psoasis (PP). The anti-HSP65IgG levels were 0.174(〕SY.+-.〔)0.032 for PGA (n=4), 0.101(〕SY.+-.〔)0.053 for PV (n=12) and 0.087(〕SY.+-.〔)0.025 for PP (n=6), showing significant changes between PGA and others (p=0.0165, PGA - PV ; p=0.001, PGA - PP).Elevation of anti-HSP65IgG was found in relation to bacterial infection-induced psoriatic and leukotactic inflammation in the skin. Less