Effects of Volatile Anesthetics on the Intracellular Ca^<2+> Concentration

研究了氟烷对离体豚鼠肝细胞内Ca^<2+>浓度（[Ca^<2+>]i）的影响。肝细胞在含Ca^<2+>的Hank’s溶液中孵育时，提示细胞死亡的台色蓝摄取为13.5<正负>3.41%，对照组[Ca^<2+>]i为158<正负>73.5nM。通过氟烷暴露，台盼蓝吸收和[Ca^<2+>]i呈浓度依赖性增加。肝细胞在排除Ca^<2+>的Hank’s溶液中孵育时，对照组台色蓝摄取为31.2<正负>6.40%,[Ca^<2+>]i为249<正负>27.5nM。氟烷暴露后，台盼蓝吸收呈浓度依赖性增加，而[Ca^<2+>]i降低。从这些结果来看，实验1和2中台盼蓝摄取的增加表明在培养基中有或没有Ca^<2+>的氟烷的肝毒性。生理状态下肝细胞[Ca^<2+>]i的升高表明氟烷对肝细胞细胞膜的直接损伤。

The effects of halothane on the intracellular Ca^<2+> concentration ([Ca^<2+>]i) of isolated guinea pig hepatocytes were investigated .When hepatocytes were incubated in Hank's solution containing Ca^<2+>, trypan blue uptake which indicates death of cell was 13.5<plus-minus>3.41%, and [Ca^<2+>]i was 158<plus-minus>73.5nM in control.By halothane exposure, both trypan blue uptake and [Ca^<2+>]i were increased in concentration-dependent fashion.When hepatocytes were incubated in Hank's solution excluded Ca^<2+>, trypan blue uptake was 31.2<plus-minus>6.40%, and [Ca^<2+>]i was 249<plus-minus>27.5nM in control.By halothane exposure, trypan blue uptake was increased in concentration-depandent fashion, while [Ca^<2+>]i was decreased.From these results, the increases of trypan blue uptake in experiments 1 and 2 indicate the hepatotoxicity of halothane with or without Ca^<2+> in medium. The increase of [Ca^<2+>]i of hepatocytes in physiological condition indicate the direct injury of halothane to hepatocyte cell membrane.