Research on the pyrenoid in Chlamydomonas

在低CO_2条件下，衣藻叶绿体中的类芘含量增加了约3倍。用蔗糖密度梯度离心的方法对hgc12固定的类黄嘌呤细胞进行离心，发现适应低CO_2条件的细胞中类黄嘌呤相对较重。类pyrenox的平均蛋白质含量也显示了相同的结果。在低CO_2条件下，Rubisco的两个小亚基基因之一rbcS1的转录量低于高CO_2条件下的转录量，而Rubisco的另一个小亚基基因rbcS2的转录量几乎相同。在氯霉素存在的情况下，仍观察到类pyrenox的发展。

The pyrenoid, which is located in chloroplast of algae, increases under low CO_2 conditions by about 3 times in Chlamydomonas. Sucrose density gradient centrifugation of the pyrenoid fixed with HgC1_2 showed relatively heavier pyrenoid in the cells adapted to low CO_2 condition. Average protein content of the pyrenoid showed the same result. During the adaptation to low CO_2 concentration where the pyrenoid developed, rbcS1, one of two genes of small subunit of Rubisco, was transcribed less than that in high CO_2 condition, while rbcS2, the other Rubisco gene, was at almost the same level. The development of the pyrenoid was still observed in the presence of chloramphenicol.