Effects of yeast and plant protoplast cells with

酵母细胞表面覆盖着坚硬的不溶于水的型多糖。由此推测，在酵母原生质细胞外包覆类似多糖对原生质细胞的稳定性有影响。本研究发现，水溶性β -1,3-葡聚糖（schizophyllan）对酿酒酵母原生质体细胞的再生促进作用达1000倍左右，β -1.3-葡聚糖具有形成三螺旋结构的特点。在对酿酒酵母原生质体细胞再生的影响方面，也认识到三螺旋结构对产生这种影响的重要性。这表明具有其他糖基键的多糖没有效果。已知-1,3-葡聚糖具有增强免疫活性的能力，其生物活性的出现与三螺旋结构密切相关。因此，人们认为这两种生物活动的产生机制是相似的。为了详细研究其作用机制，应该从β -1,3-葡聚糖的受体位点入手。发现与酿酒酵母细胞壁多糖结构相似的多糖对酵母原生质体细胞的再生有较好的效果，并对植物细胞壁多糖相关的多糖进行了大量的研究。以烟草原生质体细胞为例，发现琥珀聚糖和与水溶性-1,4-葡聚糖相关的黄原胶对烟草原生质体的稳定性有影响。

Yeast cells are covered with hard and water-insoluble type polysaccharides. So it was estimated that a coating yeast protoplast cells with a similar polysaccharide effected on the stability of the protoplast cells. In this work, it was found that water-soluble beta-1,3-glucan (schizophyllan) promoted the regeneration of protoplast cells of S.cerevisiae (Sake yeast) about one thousand times.beta-1.3-Glucans are characteristic of forming triple helix structures. In the case of the effect on the regeneration of protoplast cells of S.cerevisiae, it was also recognized an importance of the triple helix structure to appear the effect. This was suggested that polysaccharides having other glycosyl linkages were not effective. It has been known that beta-1,3-glucans have an ability of enhancing an immune activity and that the appearance of the biological activity correlated strongly with the triple helix structure. So it was thought that both biological activities have similar mechanisms for appearing of the activities. For studing in detail the mechanism, it should be investigated a recepter sites of beta-1,3-glucans.It was found that the polysaccharide having similar structure with cell wall polysaccharide of S.cerevisiae was effective on the regeneration of the yeast protoplast cells, and it was investigated lots of polysaccharides related cell-wall-polysaccharide of plants. On the case of plotoplast cells of tabacco, it was found that succinoglycan and xanthan gum related to water-solublebeta-1,4-glucans showed the effects on stabilities of protoplast of tabacco.