The behavior of the trans-Golgi networks in plant cells.

采用冷冻替代法和免疫电镜研究了植物细胞中作为囊泡转运分选位点的反式高尔基网络的行为。研究了tgn在蓝藻（Rhodophyceae）、Brachiomonas、Trebouxia、Ankistrodesmus、Pediastrum、Scenedesmus和Botryo-coccus（吊兰科）、苔藓（苔藓）、Tradescantia（高等植物）的花粉和花粉管中的存在和结构，以了解高等植物组织细胞中很少发现的tgn。在除花粉管外的所有细胞中均可见tgn。因此，可以认为tgn普遍存在于从狐猴科到高等植物的细胞中。在上述细胞中，乳杆菌球菌中的TGN在结构上是最大的，因此我们在整个细胞周期中详细研究了乳杆菌球菌中TGN的结构和行为。网格蛋白包被的囊泡总是附着在tgn上。tgn在细胞分裂过程中形成参与隔膜形成的囊泡，在细胞分裂后产生参与液泡形成的多泡体。来自高尔基体的小泡似乎通过tgn被运送到它们的目的地。我们先后制备了高尔基- tgn抗体和内质网抗体。我们正在将这些抗体应用于免疫电子显微镜，以检查与tgn相关的蛋白质分子的运动。

The behavior of the trans-Golgi networks (TGNs) in plant cells which are the sorting site of vesicle transport were examined by freeze substitution method and immuno-electron microscopy.The existence and the structure of TGNs in Cyanidium (Rhodophyceae) , Brachiomonas, Trebouxia, Ankistrodesmus, Pediastrum, Scenedesmus and Botryo-coccus (Chlorophyceae) , Bryum (Moss) , pollens and pollen tubes of Tradescantia (higher plant) were studied to get information about the TGNs which have little known in the tissue cells of higher plants. In all cells except for the pollen tubes of Tradescantia, TGNs could be observed. Accordingly, it can be suggested that the universal occurrence of the TGNs in cells from Rodophyceae to higher plants.The TGNs in Botryococcus was the biggest in structure among the cells listed above, so that the structure and the behavior of the TGN in Botryococcus were studied in ditail throughout the cell cycle. Clathrin-coated vesicles were always attached to the TGNs. The TGNs formed the vesicles which participated in the septum formation during cell division and produced multi-vesicular bodies which participated in the vacuole formation after cell division. Vesicles derived from Golgi bodies seemed to be transported to their distination sites via TGNs.The antibodies against to the Golgi-TGN and to the endoplasmic reticulum were successively produced by us. We are applying these antibodies to the immuno-electron microscopy to examine the movement of protein molecules associated TGNs.