gene expression induced by UV-B Light Study on gene expression induced by UV-B light

(1)将胡萝卜pal-10全长启动子（-2335）与荧光素酶基因（luciferase）融合，通过电穿孔法瞬时转化胡萝卜原生质体，检测UV-B光诱导的启动子活性（luciferase）。Pal-10基因启动子的表达是UV-B特异性的，并且在完整细胞中证实了UV-B光感受器参与pal-10基因的调控。(2)使用与CaMV 35 S核心启动子（~ 90）融合的不同长度的启动子片段检测UV-B特异性顺式元件。在-395和-190之间获得了两个元件，它们的共有序列是T（A）XTCXCCAACCC（A）。(3)然而，在-570处截短的含有UV-B响应顺式元件的启动子不显示UV-B响应。(4)pal-10启动子的正确表达需要UV-B响应顺式元件和上游（-2335 ~-570）和下游（-145 ~+145）序列。

(1) Carrot pal-10 full length promoter (-2335) fused to reporter gene (luciferase) was transiently transformed to carrot protoplasts by electroporation, and promoter activity (luciferase) induced by UV-B light was examined. Pal-10 full length promotor expression was UV-B specific and involvement of UV-B photoreceptor in regulation of pal-10 gene is satisfied as shown in intact cells.(2) UV-B specific cis element was examined using various length promoter fragments fused to CaMV 35S core promoter (-90). Two elements were obtained between -395 and -190 and their consensus were T (A) XTCXCCAACCC (A).(3) However, truncated promoter at -570, containing UV-B responsive cis-elements did not show UV-B response.(4) Both UV-B responsive cis-element and up (-2335 to -570) and down (-145 to + 145) stream sequences are necessary for correct expression of pal-10 promoter.