転移性を獲得した細胞の軟寒天中の行動と細胞接着及び細胞骨格の関係

在琼脂集落形成条件下，研究了肌动蛋白微丝在ras/mycSFME和r/MHM-SFME-1两种细胞系中的分布。后者由前者衍生而来，在宿主动物中表现出高度的转移潜能，而前者显示出较低的活性。用人c-Ha-ras和小鼠c-myc基因转化这两种细胞，并在软琼脂中形成集落。R/MHM-SFME-1克隆由松散排列的细胞组成，周围有散在的卫星细胞，而ras/mycSFME克隆由致密的细胞组成。罗丹明-鬼笔花素染色显示两种细胞的微丝主要分布在细胞周边。在此条件下，ras/mycSFME细胞的微丝分布较r/MHM-SFME-1细胞丰富。Ras/mycSFME细胞的活性微丝主要分布在细胞与细胞接触的区域，而r/MHM-SFME-1细胞则在细胞表面形成细小的网状结构。然而，当这些细胞被培养在纤维连接蛋白涂层的培养皿中时，它们表现出纤维母细胞的外观，并在培养皿上很好地扩散。然后，在软琼脂凝胶的三维培养中可以显示两种细胞转移潜能的表型差异。

Distribution of actin-microfilaments in two cell lines, one is ras/mycSFME and the other is r/mHM-SFME-1, was studied in a colony forming conditions in agar gel. The later, which derived from former, shows highly metastatic potentials in host animals whereas the former shows lower activity. Both cells were transformed by human c-Ha-ras and mouse c-myc genes and produced colonies in soft agar. The r/mHM-SFME-1 colonies were consisted of loosely packed cells with dispersed satellite cells around the colony whereas ras/mycSFME ones were made of fairly compacted cells. Rhodamine-phalloidin staining showed that microfilaments of the two cells were present mainly at cell periphery. The distribution of microfilaments in this condition was more abundant in ras/mycSFME cells than in r/mHM-SFME-1 cells. The cell-cell adhesion developed well in former than in the later one.Actin-microfilaments were detected mainly at the cell-cell contacted area in ras/mycSFME cells, whereas they were forming thin network under the surface of r/mHM-SFME-1 cells. When these cells were cultured on fibronectin coated dishes, however, they expressed a fibrobrastic appearance and spread well on the dishes. Then, the phenotypic difference in metastatic potentials of the two cells could be demonstrated in the three-dimensional cultures in soft agar gel.