Aberrant function and expression of gap junction proteins (connexins) during carcinogenesis

(1)小鼠皮肤多阶段癌变过程中间隙连接蛋白（connexins[Cx]）的异常表达（癌变16:1287-1297,1995）(a) Cx26和Cx43在正常和周围非肿瘤表皮中表达差异。(b)在乳头瘤中，cx26和Cx43经常共存于相同的间隙连接斑块中。(c)在鳞状细胞癌中，与周围非肿瘤表皮相比，Cx26和Cx43的表达均显著降低。(d) Cx26的表达随着癌细胞形态分化程度的降低而降低。(e)侵袭部位的鳞状细胞癌显示Cx26和Cx43明显减少。(f)在转移到淋巴结的鳞状细胞癌中，表达Cx26，但表达Cx43的癌细胞很少。(g)即使在侵袭和转移部位，E-cadherin在癌细胞之间的质膜上的定位也保持不变。(2)人食管癌细胞中连接蛋白的表达、功能和定位[J] .中国食管癌杂志，1994,(1):Cx26和Cx43在正常人食管组织中的表达。(b)大多数人食管癌细胞系表达的Cx26和Cx43 mrna量低于正常人食管癌组织。(c) Cx26和Cx43 mrna和蛋白的共表达仅在两种表现出高水平GJIC和非进行性肿瘤发展的细胞系中观察到。(d) E-cadherin在所有细胞系中均有表达。(3)肿瘤启动子DDT对大鼠肝间隙连接细胞间通讯的影响（cancer genesis 15: 517-542,1994）DDT在体内剂量依赖性地抑制肝间隙连接细胞间通讯，改变Cx32和Cx26蛋白的表达和定位。(4)创面愈合过程中连接蛋白在仓鼠口腔上皮中的表达变化在创面愈合过程中，连接蛋白及其转录本的表达和定位发生了巨大的变化。

(1) Aberrant expression of gap junction proteins (connexins[Cx]) during multistage mouse skin carcinogenesis (Carcinogenesis 16 : 1287-1297,1995)(a) Cx26 and Cx43 were differentially expressed in normal and surrounding non-tumorous epidermis. (b) In papillomasCx26 and Cx43 were frequently co-localized in the same gap junction plaques. (c) In squamous cell carcinomas, the expression of both Cx26 and Cx43 significantly decreased compared with surrounding non-tumourous epidermis. (d) The expression of Cx26 was reduced as cancer cells became morphologically less differentiated. (e) Squamous cell carcinomas at invasiv sites showed clear reduction of Cx26 and Cx43. (f) In squamous cell carcinomas metastasized into lymhph nodes, Cx26 was expressed, but few carcinoma cells expressed Cx43. (g) The localization of E-cadherin on the plasma membrane between cancer cells was maintained even at invasive and metastatic sites.(2) Aberrant expression, function and localization of connexins in human esophageal carcinoma cell lines (J Cancer Res Clin Oncol 120 : 445-453,1994)(a) Normal human esophageal tissue expressed both Cx26 and Cx43. (b) Most of the human esophageal carcinoma cell lines expressed lower amounts of Cx26 and Cx43 mRNAs than normal human esophageal tissues. (c) The co-expression of Cx26 and Cx43 mRNAs and proteins was observed only in two cell lines that showed a high level of GJIC and non-progressive tumor development. (d) E-cadherin was expressed in all cell lines.(3) Effect of a tumour promoter, DDT,on hepatic gap junctional intercellular communication in rats (Carcinogenesis 15 : 517-542,1994)DDT inhibited hepatic gap-junctional intercellular communication in vivo dose-dependently and changed Cx32 and Cx26 protein expression and localization.(4) Changes in the expression of connexins in hamster oral epithelium during wounnd healingDuring wound healing, the expression and localization of connexin proteins and transcripts were changed drastically.