In vivo effects of sIL-IR and sIL-4R on IgE production and regulation of allergic reaction

研究了il - 4r和IL-1R对小鼠GVHD模型体内和体外IgE生成的影响。il - 4r对IL-4依赖性细胞株（ctl -2）的影响：il - 4r和IL-4共同培养，ctl -2受到抑制。然而，感染Nb的小鼠的IgE产生。体内注射sIL-4R无抑制作用。sIL-1R的作用：骨髓细胞（BMC）加脾脏细胞（C57BL/6或BALB/c）辐照（BALB/c × C57BL/6）F1（CBF1）诱导GVH反应导致GVHD （BMT/GVHD）。供体BALB/c诱导慢性GVHD， C57BL/6诱导急性GVHD。在小鼠急性GVHD模型中，分别用sIL-1R和sIL-4R治疗GVHD小鼠，可改善GVHD小鼠肝脏疾病的器质性衰竭、脾肿大和T细胞成熟的免疫功能障碍。在我们的研究中：1)sIL-4R能有效抑制IgE的产生和抑制CTLL-2细胞。2) sIL-1R对小鼠急性GVHD有改善作用。

The effect of sIL-4R and IL-1R were carried out on IgE production or murine GVHD models in vivo and in vitro.The effect of sIL-4R : IL-4 dependent cell line (CTLL-2) were cultured with sIL-4R and IL-4, CTLL-2 was inhibit. However, IgE production in mice infected with Nb. was not suppressive by the injection of sIL-4R in vivo.The effect of sIL-1R : Induction of GVH reaction in irradiated (BALB/c x C57BL/6)F1(CBF1) with bone marrow cells (BMC) plus spleen cells of (C57BL/6, or BALB/c) lead to GVHD (BMT/GVHD). The use of donor as BALB/c induced chonic GVHD whereas taht as C57BL/6 induced acute GVHD.When in murine acute GVHD model mice were treated with sIL-1R but sIL-4R, oranic falure of liver disease, splenomegaly and Immunodysfuncitons of T cell maturations were improved in GVHD mice.In our studies :1) sIL-4R were effective to suppression of IgE productions and inhibition of CTLL-2 cells.2) sIL-1R were effective to improvement of acute GVHD in mice.