STUDIES ON CLINICAL APPLICATION OF LASER PHOTODYNAMIC TREATMENT TO KERATOTIC SKIN DISEASES

先前研究了角化性皮肤病移植嵌合体的功能形态，并对这些移植嵌合体进行了增殖和分化的建模。这些移植嵌合体也可作为角膜性皮肤病激光光动力治疗（PDT）的材料。本研究对激光PDT进行了临床评价。在激光PDT中，使用了红宝石激光器和酞菁和5-氨基乙酰丙酸作为光敏剂。研究结果如下：(A)寻常型银屑病的研究结果：采用皮肤移植法（STM）、皮肤包埋法（SETM）和皮肤开孔移植法（SFTM）将寻常型银屑病病变移植到裸鼠体内。一篇题为“开窗移植法移植寻常型银屑病病变裸鼠皮下组织的超微结构”的论文目前正在《皮肤病学杂志》上发表。研究了经SFTM移植的病变为激光PDT提供了有用的材料。激光PDT治疗寻常型银屑病的临床应用。(B)角化性皮肤癌的发现：(1)以C_3H小鼠的实验性皮肤癌为材料进行激光PDT。结果先前有报道，激光PDT后，在真皮上部发现化脓性癌细胞，水肿和出血。电镜下，癌细胞细胞核变形，线粒体嵴消失。观察到线粒体变形是激光PDT后的初始变化。(2)将皮肤癌和恶性黑色素瘤作为临床材料。对皮肤癌的研究报告题为“裸鼠汗腺癌和激光PDT后癌细胞的超微结构”。激光PDT后癌细胞的初步变化为线粒体变形。细胞核和细胞器在细胞质中依次发生变化。真皮部分出现水肿和出血。肿瘤团块退化。利用移植嵌合体和体外材料对恶性黑色素瘤病变的研究仍在继续。(3)在Bowen病的病变中，报道了激光PDT的结果，结果显示激光PDT的临床应用对Bowen病的病变治疗是有益的。此外，在活性氧领域对激光PDT作用机理的研究也在继续。结论激光PDT是治疗角化性皮肤病的有效方法

Functional morphology was previously investigated in transplantation chimeras of keratotic skin diseases, and these transplantation chimeras underwent modeling on proliferation and differentiation. These transplantation chimeras of keratotic skin diseases were also used as materials for laser photodynamic treatment (PDT) of keratotic skin diseases. Clinical evaluation of laser PDT was perfomed by the present investigation. In laser PDT,ruby laser and phthalocyanine and 5-aminolevulinic acid as photosensitizer were used. The results are as follows ; (A) The findigs of psoriasis vulgaris : Psoriasis vulgaris lesions were transplanted into nude mice by the skin transplantation method (STM), skin embedding transplantation method (SETM), and skin fenestrating transplantation method (SFTM). A paper entitled "Ultrastructure of the lesions of psoriasis vulgaris transplanted into the subcutaneous tissue of nude mice, by the fenestrating transplantation method" is currently in press in the Journ … More al of Dermatology. Lesions transplnted by SFTM were useful materials for laser PDT was investigated. Clinical application of laser PDT on psoriasis vulgaris lesions. (B) The findings of keratotic skin cancer : (1) Experimental skin cancer in C_3H mice was used as materials for laser PDT.The results have previously been reported, and after laser PDT,pyknotic cancer cells, edema and bleeding were revealed in the upper dermal portion. Electron-microscopically, nuclei of cancer cells were deformed and mitochondrial cristae disappeared. It was observed that the mitochondrial deformation was the initial change after laser PDT.(2) Skin cancer and malignant melanoma were used as clinical materials. Investigation of skin cancer was reported under the title "Ultrastructure of sweat organ cancer in nude mice and cancer cells after laser PDT". The findings of cancer cells after laser PDT showed mitochondrial deformation as the initial changes. Nuclei and organelles changed successively in the cytoplasm. In the dermis, edema and bleeding were observed. The tumor masses degenerated. Investigation of malignant melanoma lesions using transplantation chimeras and in vitro-materials is continuing. (3) In the lesions of Bowen's disease, the results of laser PDT were reported, and findings revealed that clinical application of laser PDT was useful in treating lesions of Bowen disease. Furthermore, the investigation of the action mechanisms of laser PDT are continuing in the field of active oxygen.In summary, it was concluded that laser PDT was a useful treatment for the keratotic skin diseases Less