LOCALIZATION OF CORNIFIN IN EPIDERMAL KERATINOCYTE AND ITS EXPRESSION IN SKIN DISEASES

本研究通过北方印迹杂交、免疫印迹分析和荧光化学方法比较了角蛋白α和β在几种组织中的表达。通过北方印迹杂交，在正常表皮、银屑病表皮、皮肤鳞状细胞癌和口腔牙龈上皮中检测到0.8kb的角蛋白α转录本。1.1 kb的角蛋白β转录只在口腔牙龈上皮和疣状皮肤癌中检测到，但在正常和银屑病表皮，也不是在皮肤鳞状细胞癌。免疫印迹分析显示，SQ 37 C-Ab的人角蛋白α/SPRR 1，识别一个14.0 kD的蛋白质的提取物从分化的正常人表皮角质形成细胞，正常表皮从头皮和鞋底，银屑病表皮。该抗体可识别正常牙龈上皮提取物中14.0、16.0和26.0kD蛋白，识别皮肤疣状癌提取物中14.0、16.0和25.0kD蛋白以及表观分子量小于14.0kD的蛋白。角蛋白β的CL 12 PEPB-Ab不与表皮组织提取物反应，但强烈识别牙龈上皮组织提取物中的26.0 kD蛋白。对正常皮肤和各种皮肤病的样本的分析显示：1）角蛋白α在表皮的颗粒层、毛囊的内根鞘以及口腔和食管上皮的上鳞状层中表达，2）角蛋白α在增生性粒细胞缺乏性病变（如在寻常型银屑病中观察到的）和在颗粒过多性病变（如在扁平苔藓中观察到的）中均上调，3）cornifin β在正常口腔和食管上皮中表达，在皮肤和皮肤病中不表达，疣状癌除外。

In this study, the expression of cornifin alpha and beta was compared in several tissues by Northern blot hybridization, immunoblot analyzes, and immunohistochemstry. By Northern blot hybridization, an 0.8 kb cornifinalpha transcript was detected in normal epidermis, psoriatic epidermis, squamous cell carcinoma of the skin, and oral gingival epithelia. The 1.1 kb cornifin beta transcript was detected only in oral gingival epithelia and verrucous carcinoma of the skin, but neither in normal and psoriatic epidermis nor in squamous cell carcinoma of the skin. Immunoblot analysis revealed that SQ37C-Ab for human cornifin alpha/SPRR1, recognized a 14.0-kD protein in extracts from differentiated normal human epidermal keratinocytes, normal epidermis from scalp and sole, and psoriatic epidermis. The antibody recognized 14.0,16.0, and 26.0 kD proteins in extracts from normal gingival epithelia, and 14.0,16.0,25.0 kD proteins, and some proteins with apparent molecular weight smaller than 14.0 kD in extracts from verrucous carcinoma of the skin. The CL 12PEPB-Ab for cornifin beta did not react with extracts from epidermal tissues but strongly recognized a 26.0 kD protein in extracts from gingival epithelial tissues. Analyzes of specimens from normal skin and various skin diseases showed that 1) cornifin alpha was expressed in the granular layr of the epidermis, in the inner root sheath of the hair follicle, and in the upper squamous layrs of oral and esophageal epithelia, 2) cornifin alpha was up-regulated both in hyperplastic, agranulocytic lesions as observed in psoriasis vulgaris and in hypergranulotic lesions as observed in lichen planus, 3) cornifin beta was expressed in normal oral and esophageal epithelia but not in skin and skin diseases, except for verrucous carcinoma.