Role of stress proteins (HSP65) and dendritic cells in inflammatory skin diseases

身体远处的慢性和潜伏性感染病灶可能引发皮肤的各种炎症性疾病。它被解释为“局灶性感染”，但没有解释确切的机制。我们注意到这个过程包含了各种有趣的主题，这导致了我们现在的项目。免疫遗传学上保守的应激蛋白或热休克蛋白（HSPs）被认为在寄生微生物和被感染的宿主人类中都是诱导的。这可能导致宿主对细菌的防御，另一方面，偶尔会诱导免疫调节，因为细菌热休克蛋白具有高度的免疫原性，是宿主免疫监视的目标，而感染应激后诱导的哺乳动物热休克蛋白能够被宿主的相同免疫机制识别。我们用ELISA技术研究了各种炎症性皮肤病中分枝杆菌HSP65的抗体水平，以便将其与所谓的“局灶性感染”联系起来。与正常组比较，掌性脓疱病、银屑病、荨麻疹和带状疱疹患者对麻风分枝杆菌HSP65的IgG抗体水平升高。银屑病患者急性口状银屑病患者抗hsp65 IgG显著升高，寻常型银屑病和脓疱型银屑病患者抗hsp65 IgG无显著升高。在掌足底脓肿患者中，感染灶患者的抗hsp65 IgG水平明显高于无感染灶患者。我们进一步研究了皮肤疾病患者对葡萄激酶的抗体水平，葡萄激酶是由感染性金黄色葡萄球菌产生和分泌的抗原和纤溶蛋白。我们发现抗葡萄激酶是葡萄球菌感染的一个有用的指标，并且是一个独立于ASLO和ASK的参数，这是链球菌感染的指标。伴有感染灶的银屑病和掌足底脓肿患者抗葡萄激酶IgG明显升高。最近，我们用免疫组织化学方法研究了真皮树突状细胞的标记物，如XIIIa因子、血栓调节蛋白和CD34。每个标记都显示出独特的细胞群和分布在发炎的皮肤组织中。这些树突状细胞与单核吞噬细胞的生物学意义目前正在研究中。少

Chronic and latent infectious focus in a distant part of the body may trigger diverse inflammatory disorders in the skin. It has been interpreted as "focal infection" without explainion of the precise mechanism. We noticed that this process includes various interesting subjects, that lead us to the present projict. Immunogenetically well-conserved stress proteins or heat shock proteins (HSPs), are considered to be induced in both parasitic microorganisms and infected host humans.This may result in host-defense to bacteria and, on the other hand, occasional induction of immunomodulation, since bacterial HSPs are highly immunogenic and targetted by immune surveilance of hosts, while mammalian HSPs indued after infectious stress are able to be recognized by the same immune mechanism of the host. We have investigated antibody level to mycobacterial HSP65 with ELISA technique in various inflammatory skin dieases in order to correlate this with so-called "focal infection". In comparison to n … More ormal group, patients with palmoplanter pustulosis, psoriasis, urticaria, and herpes zoster showed elevated IgG antibody level to HSP65 from Mycobacterium leprae. Among patients with psoriasis acute guttate psoriasis showed significant increase in anti-HSP65 IgG,while psoriasis vulgaris and psoriasis pustulosa showed no significant increase. Among patients with palmoplantar pustulosis, patients with infectious focus showed significant increase in anti-HSP65 IgG,compared to those without it.We further investigated antibody level to staphylokinase, that is antigenic and fibrinolytic protein produced and secreted by infectious Staphylococcus aureus, in patients with the skin disorders. We found that anti-staphylokinase is a useful indicator of staphylococcal infection, and an independent parameter from ASLO and ASK,that are indicators of streptococcal infection.Patients with psoriasis and palmoplantar pustulosis associated with infectious foci showed significant increase in anti-staphylokinase IgG.Recently we immunohistochemically investigated dermal dendritic cells with markers such as Factor XIIIa, Thrombomodulin, and CD34. Each marker showed unique cell population and distribute in the inflamed skin tissue. Biological significance of those dendritic cells in relation to mononuclear phagocytes are now under investigation. Less