The effect of TGF-alpha and EGF to the liver regenerat

采用口服ccl_4和苯巴比妥制备肝硬化大鼠模型。该方法的死亡率为24.1%，存活大鼠几乎全部为完全肝硬化。我们对该大鼠进行70%肝切除，并测量^ 3h -硫胺的掺入。正常大鼠为37.3<正负>3.4cpm/gDNA，肝硬化大鼠为15.6<正负>3.4cpm/gDNA。因此，该模型的杠杆再生明显下降。接下来，我们检测了生长因子[转化生长因子a (tgf - α)，表皮生长因子（EGF），胰岛素]对70%肝切除后肝脏再生的影响。正常大鼠肝感染70%皮下注射tgf - α不增加^ 3h -硫胺掺入。EGF也有相同的结果。肝硬化大鼠皮下注射tgf - α可明显增加^ 3h -噻咪啶的掺入。EGF与胰岛素联合注射结果相同。tgf是由肝脏产生的，所以正常大鼠有足够的tgf，但肝硬化大鼠缺乏tgf。所以注射tgf -增加了肝脏再生。这些生长因子的支持治疗可用于肝硬化肝切除术，以增加肝脏再生。

The liver cirrhosis rat model was made by the oral intake of C CL_4 and phenobarbital. In this method, the mortality rate was 24.1%, and almost all the survived rats had compelete cirrhotic liver. We performed the 70% liver resection on this rats, and measured the ^3H-thimidine incorporation. This was 37.3<plus-minus>3.4cpm/gDNA in normal rat, whereas 15.6<plus-minus>3.4cpm/gDNA in liver cirrhosis rat model. So marked decrease of lever regeneration in that model was manifested. Next we examined the effect of growth factors [Transformining growth factor a (TGF-alpha), epidermal growth factor (EGF), insulin] to the liver regeneration after 70% liver resection. Subcutaneous injection of TGF-alpha on normal rat that was hepatected 70% did not increased the ^3H-thimidine incorporation. That of EGF was same result. But subcutaneous injection of TGF-alpha on the liver cirrhosis rat markedly increased the ^3H-thimidine incorporation. And the combined injection of EGF and insulin showed the same result.TGF-is made fromthe liver, so normal rat have enough amount of it, but liver cirrhosis rat lacks it. So the injection of TGF-alpha increased the liver regeneration.Support therapy of these growth factors may be used in the liver resection of cirrhosis liver to increase the liver regeneration.