Effects of the iontophoretically applied drugs using multi-barrel glass micro-pipette on the neural activities of the sacral micturition center in the rat.

在乌拉坦麻醉的Wistar大鼠上，观察了离子导入神经递质对脊髓Onuf核神经活动的影响。电刺激阴部神经(持续时间30微秒，10~20微安，双脉冲，间隔5ms，频率1~20赫兹)，记录逆行诱发电位。采用稳定潜伏期和对10赫兹双脉冲刺激的反应作为逆行反应的标准。将5根充满桥胺天蓝(记录电极)、谷氨酸、去甲肾上腺素、5-羟色胺或生理盐水的Barret玻璃微电极插入脊髓，观察其对阴部神经条件性电刺激引起的自发放电和/或神经反应的药理作用。在31个被测神经元中，有5个神经元被谷氨酸离子导入兴奋，1个神经元被5-羟色胺抑制。去甲肾上腺素对受试神经元有影响。由于Onuf核记录技术的困难，本研究得到的结果并不是决定性的。然而，这项研究中发展的方法学对于各种递质和药物对排尿反流的神经机制的脊髓作用的特征研究应该是非常有用的。

Effects of iontophoretically injected neurotransmitters on the neural activities of Onuf's nucleus located in the sacral spinal cord were examined in uerthane anesthetized Wistar rats. The pudendal nerve was electrically stimulated (30mus in duration, 10-20muA,twin pulses : 5 ms interval at 1-20Hz) and the antidromically evoked potential was recorded. Stable latency and responses to the twin pulse stimulation at 10Hz was adopted the criteria for the antidromic response. Five barret glass glass micropipette filled with pontamine sky blue (recording electrode), glutamate, noradrenalin serotonin or saline was inserted into the sacral spinal cord and their pharmacological effects on the spontaneous discarges and/or neural responses to the conditioning electrical stimulation of the pudendal nerve were examined. Five of 31 neurons tested were excited by the iontophoretical application of glutamate and one neuron was inhibited by serotonin. Noradrenalin had to effects on the neuron tested. Because of the difficulties of recording technique from the Onuf's nucleus, the results obtained in the present study are not conclusive. However, the methodology developed in this study should be quite useful for the feature studies on the spinal action of the various transmitters and drugs on the neural mechanisms of micturition reflux.