Mechanism of expression and biological function of sulfated glycolipids in the endometrium

本研究的目的是（1）分析子宫内膜中磺基转移酶（ST）和芳基硫酸酯酶（阿萨）的定量变化，（2）定位硫酸化糖脂，（3）阐明硫酸化糖脂对子宫内膜细胞粘附的贡献。1.当在正常子宫内膜中测量时，与增殖期相比，分泌期的ST活性增加，而与分泌期相比，增殖期的阿萨活性增加。这些结果表明，子宫内膜中硫酸糖脂表达的变化涉及ST和阿萨活性的改变。使用35 S-示踪剂评估来自子宫内膜癌、子宫颈癌和卵巢癌的肿瘤株中硫酸化糖脂的表达。硫酸化糖脂在子宫内膜癌来源的肿瘤株中特异性表达，并且它们的组成在不同株之间变化。硫脂 ...更多信息 主要由来源于高分化癌的石川菌株表达，而乳糖苷脂分别由来源于中等分化癌和低分化癌的SNG-M和HEC 108菌株表达，表明分化程度与硫酸化糖脂表达之间的相关性。ST活性特异性地检测到来自子宫内膜癌的肿瘤株。阿萨活性在子宫内膜癌来源的菌株中也倾向于更高，但与其他癌症相比没有显着增加。提示ST段活性增强是导致子宫内膜癌组织中糖脂表达增加的重要原因，是子宫内膜癌的生物学特征之一。2.用一种新的抗硫酸化糖脂单克隆抗体--硫苷脂（sulfatide），对子宫内膜中的硫酸化糖脂进行了电化学定位。糖脂主要存在于腺上皮细胞中。3.为了评价子宫内膜硫酸化糖脂表达与性激素之间的关系，研究了雌激素和孕激素对石川品系的影响。ST活动增加约2倍，在雌激素加孕酮的存在下。这一发现表明性激素通过ST活性的变化参与介导子宫内膜癌硫酸糖脂表达。少

The objectives of the present study were to (1) analyze quantitative chages of sulfotransferase (ST) and arylsulfatase (ASA) in the endometrium, and (2) to localize sulfated glycollpids, and (3) to elucidate the contribution of sulfated glycolipids to cell adhesion in the endometrium. 1. When measured in the normal endometrium, ST activity was increased in the secretory phase compared to the proliferative phase, whilst ASA activity was increased in the proliferative phase compared to the secretory phase. These findings indicated that the changes of sulfated glycolipid expression in the endometrium involved alterations of ST and ASA activity. Expression of sulfated glycolipids in tumor strains derived from cancer of the endometrium, cancer of the uterine cervix, and ovarian cancer was assessed using a 35S-tracer. Sulfated glycolipids were specifically expressed in tumor strains derived from cancer of the endometrium and their composition varied between the different straings. Sulfatide … More was mainly expressed by the Ishikawa strain derived from well-differentiated cancer, whilst lactosylfatide was expressed by the SNG-M and HEC108 strains derived from moderately and poorly differentiated cancer, respectively, suggesting correlation between the degree of differentiation and the expression of sulfated glycolipids. ST activity was specifically detected in tumor strains derived from cancer of the endometrium. ASA activity also tended be higher in strains derived from cancer of the endometrium, but showed no significant increase compared to other cancers. These dindings suggested that enhanced ST activity was responsible for the expression of silfated glycolipids and was a biological characteristic of cancer of the endometrium. 2. A new monoclonal antibody directed againist a sulfated glycolipid, sulfatide, was used to immunohistochemically localize sulfated glycolipids in the endometrium. Glycolipids were found to be present mainly in the glandular epithelial cells. 3. To evaluate the relationship between endometrial expression of sulfated glycolipids and sex hormones, the effect of estrogen and progesterone on the Ishikawa strain was studied. ST activity was increased about two-fold in the presence of estrogen plus progesterone. This finding suggested that sex hormones were involved in mediating the expressin of sulfated glycolipids by cancer of the endometrium through changes in ST activity. Less