Stduy on residual fluoride materials in human body by fluoride analysis

以全氟辛酸（PFOA）和氟莫西钠（FMOX）在不同血液成分中的分布为指标，研究了这些化学物质在这些成分中的掺入氟。氟的测定采用低温氧灰-气相色谱法（LOPA-GC法）。在仅由白蛋白和红细胞组成的简单系统中，PFOA和FMOX与血液系统各组分结合的时间过程与与白蛋白和红细胞结合的时间过程有很大不同。相反，在细胞悬液中，与白蛋白结合的FMOX很容易被释放。PFOA从白蛋白中缓慢释放。红细胞中PFOA和FMOX的释放不明显。结果表明，有机氟化合物在体内的运动可以通过氟化物分析来追踪，PFOA和FMOX的运动可以通过它们与白蛋白和红细胞结合的速度差来解释。骨形态发生蛋白（bone morphogenetic protein， BMP）是骨形成的重要因子。然而，形成的骨头是易碎的骨头，骨髓腔宽。氟化物加速骨形成和钙化也是众所周知的。为此，将骨基质明胶（BMG）移植到大鼠腹肌中，并通过饮水或局部应用氟化物。然后观察氟对新骨形成的影响。结果表明，通过饮用水应用氟化物可加速钙进入新骨，激活alp酶活性并进一步钙化。

The distribution of perfluorooctanoic acid (PFOA) and flomoxef sodium (FMOX) in various components of blood was investigated by taking fluoride incorporated into these components as an indicator of these chemicals. Fluoride was measured by the low temperature oxygen ashing-gas chromatography method (LOPA-GC method). The time courses of the binding of PFOA and FMOX to the components of blood system were considerably different from those of binding to albumin and erythrocytes in a simple system composed only of albumin and erythrocytes. In contrast to the fact that FMOX bound to albumin in the cell suspension was easily released. PFOA was very slowly released from albumin. The release of both PFOA and FMOX from erythrocytes was little noticeable. It was shown that the movement o organic fluorine compounds in the body can be traced by the fluoride analysis, and that the movement of PFOA and FMOX can be interpreted by the velocity differece between their bindings to albumin and erythrocytes.It is well known that bone morphogenetic protein (BMP) introduces osteogenesis. However, formed bones are frangible bones with wide cavity of bone marrow. That fluoride accelerates the osteogenesis and calcification is also well known. Thus, bone matrix gelatin (BMG) was transplanted in abdominal muscles of rats and fluoride was applied via drinking water or localized application. Then, effect of fluoride application on new bone formation was observed. As results, it was shown that fluoride application via drinking water accelerates Ca incorporation into new bones, activation of ALP-ase activity and calcification more.