Studies on Changes of Urinary Protein Components and Alkaline Phosphatase as Spotrs-Related Stress Markers in Growth Phase

为了更全面地了解与运动相关的应激标志物，我们研究了剧烈运动前后的尿蛋白成分、碱性磷酸酶（ALP）水平和同工酶模式。在跑步后5 ~ 15分钟和1小时观察尿蛋白浓度和ALP活性峰值。SDS-PAGE和Western blotting结果显示，在跑步前和跑步后3 h收集的尿液中，白蛋白是主要的蛋白带。然而，除了白蛋白外，总蛋白浓度的峰值部分还可见到α 2-巨球蛋白、免疫球蛋白G和转铁蛋白。虽然观察到高分子质量的ALP水平在剧烈运动后略有增加，但运动的强度和持续时间似乎都没有对它们产生过于显著的影响。丁醇提取后，聚丙烯酰胺凝胶中观察到明显的ALP活性带，具有与肾皮质和肾髓质的相同程度的流动性。运动后尿ALP的一部分被认为来自肾脏。由于ALP现在被认为是骨代谢的标志之一，尿ALP肯定会受到更严格的审查，因为它与运动相关的压力和骨质疏松症有关。

In order to gain a fuller understanding of soprts-related stress markers, we studied urinary protein components and alkaline phosphatase (ALP) levels and isozyme patterns before and after strenous exercise. The urinary protein concentrations and ALP activity-peaks were seen 5-15 minutes and 1 hour after running. SDS-PAGE and Western blotting showed that albumin was the main protein band in the urine which was collected before and 3 houes after running. However, alpha2-macrogloblin, immunogloblin G and transferrin wer seen in the peak frction of total protein concentration, in addition to albumin. While levels of ALP of higher molecular mass were observed to increase slightly, following intense exerclse, neither the intensity nor the duration of the exercise appeared to have an overly significant effect on them.After buthanol extraction, a distinct band of ALP activity was observed in the polyacrylamide gel, having the same degree of mobility as those of the renal cortex and medulla.A portion of post-exercise urinary ALP was considered to have originated from the kidney. As ALP is now understood to be one of the markers of bone metabolism, urinary ALP will most certainly come under ever more intense scrutiny, as regards its connection to sports-related stress and osteoporosis.