MAPPING OF bg GENE IN RAT USING MICROSATELLITE MARKER.

Chediak-Higashi综合征(CHS)是一种常染色体隐性遗传病，已在人、水貂、猫和小鼠中得到报道。CHS最初被认为是一种黑素细胞、中性粒细胞和淋巴细胞含有巨大的细胞质颗粒的疾病。与人类CHS相似的小鼠的突变被从稀释的毛色中命名为“米色”。如果有CHS大鼠，它们可能对CHS的生化分析等研究有用。我们发现，滨松大学医学院保存的毛色突变株DA与人类CHS相似，命名为DA-BG。本研究试图将BG基因定位在大鼠染色体上。获得了以下结果，小鼠Cl.13与大鼠Cl.17具有可比性，因此，我们收集了8个微卫星标记(MSM)。用聚合酶链式反应方法扩增出MSM基因的6个片段。对10个近交系大鼠进行了MSM检测，发现在MSM的两个位点(催乳素：PRL和胆碱能受体，Muscaric3：ACRM)上存在DA-BG和BN的多态性，在80个回交后代中，PRL与BG连锁(30 CM)，ACRM与BG连锁(23 CM)，PRL与ACRM连锁(11 CM)。BG基因已被证实位于大鼠的Cl.17上。

Chediak-Higashi syndrome (CHS) is an autosomal recessive genetic disease that has been reported in human, mink, cats, and mice. CHS was initially recognized as a disorder in which melanocytes, neutrophils, and lymphocytes contained giant cytoplasmic granules. The mutation of mice comparable to human CHS is named "beige " from the diluted coat color. If CHS rats are available, they are probably useful for investigations such as biochemical analysisof CHS.We found that the coat color mutant DA strain maintained in Hamamatsu University School of Medicine was comparable to human CHS and it was named DA-bg. In this research we attempted to mapping bg gene on rat chromosome. The following results were obtained,The mouse Chr.13 is comparable to the rat Chr.17 so, we collected 8 of microsatellite marker (MSM). The 6 of MSM were amplified by PCR method. The 10 of inbred strains of rats were tested by these MSM and it was found polymorphism between DA-bg and BN at two (Prolactin : PRL and Cholinergic recepter, muscaric3 : ACRM) of MSM.In 80 progeny of {(DA-bg*BN)*DA-bg} backcross, it was found linkage between PRL to bg (30cM), ACRM to bg (23cM), and PRL to ACRM (11 cM). It was confirmed that the bg gene was on Chr.17 in rat.