"Expression of c-fos protein in rat brain after occlusion of superior mesenteric artery (immunohistochemical investigation)"

观察大鼠肠系膜上动脉结扎后0、2、4、6、8h脑组织c-fos表达和血浆内毒素水平的变化。在假手术大鼠脑内，c-fos免疫组织化学染色显示少数神经元细胞散在分布于下丘脑室旁核、下丘脑视上核和缰核外侧核。然而，在动脉闭塞处理的大鼠中，在不同脑区的某些人群中观察到许多c-fos阳性神经元。这些阳性细胞最早在治疗后2小时被检测到。孤束核、穹隆下器、蓝斑、迷走神经背侧运动核、下丘脑室旁核、视上核、腹内侧核、缰核外侧核、杏仁中央核等具有结扎诱导c-fos阳性神经元的区域，早期血浆内毒素水平较低，术后6h迅速升高。结果表明，某些神经系统，如边缘、下丘脑和脑干区，似乎参与了肠系膜动脉闭塞的病理生理反应。目前的神经解剖学证据可能支持这样的观点，即人类坏死性肠炎和脓毒症中的情绪变化是由于败血症相关的改变对中枢神经系统的直接影响。此外，由于脑内这种神经元的激活先于血浆内毒素水平的升高，因此内毒素以外的其他未知因素可能会对大脑产生影响。

The expression of c-fos in brain and endotoxin level in plasma were studied at 0,2,4,6,8 hours after occlusion of the superior mesenteric atery of rat. In sham-operated rat brain, a few neuronal cells strained by immunohistochemistry for c-fos were scattered in the hypothalamic paraventricular, hypoyhalamic supraoptic and lateral habenular nuclei. In arterial occlusion treated rats, however, many c-fos positive neurons were observed in certain populations of various brain regions. These positive cells were first detected as early as 2 hours after the treatment. The regions possessing occlusion-induced c-fos positive neurons include the nucleus tractus solitalius, subfornical organ, locus coeruleus, dorsal motor nucleus of vagus, hypothalamic paraventricular, supraoptic and ventromedial nuclei, lateral habenular nucleus, and central nucleus of amygdala, In these animals, the plasma endotoxin were kept at low levels during early period but were rapidly increased 6 hours afer surgery. The results indicate that certain neuronal systems such as the limbic, hypothalamic, and brainstem regions appear to be involved in a pathophysiological response to the mesenteric arterial occlusion. The present neuroanatomical evidence may support the idea that the emotional change seen in human necrotizing enteritis and sepsis is due to a direct influence of sepsis-associated alterations on the central nervous system. Since, inaddition, such neuronal activation in brain preceedes the elevation of plasma endotoxin level, other unknown factors than endotoxin may conduct the influence to the brain.