A diagnostic study of lectin binding pattern and color variation in oral mucosal epithelium on diseases of the oral mucosa

许多口腔黏膜疾病病因不明。通常临床表现为大疱性、糜烂性、角化性、溃疡性病变，因此很难进行鉴别诊断。本研究的目的是探讨WGA使用对扁平苔藓上皮细胞膜可能的影响，以及不同临床类型扁平苔藓（OLP）的颜色变化。结果如下：1)检测上皮细胞与小麦胚芽凝集素（WGA）的结合情况。在健康口腔粘膜上皮中，凝集素结合规律强，而在各种临床类型的OLP中，凝集素结合规律弱，特别是在糜烂性病变中，凝集素结合减少最多。2)采用CHROMA仪CS-100（MINOLTA）检测上皮细胞的颜色变化。颜色空间（CIE 1976 L^<**>a^<**>b^<**>）和色差（CIE 1976 DELTAE^<**>ab）被检查。与正常扁平苔藓粘膜上皮相比，L^<**>和b^<**>为减量，a^<**>为正量。色差DELTAE^<**>ab高约7级。3)采用视觉模拟量表（visual analogue scale， VAS）定量测量OLP引起的疼痛。各临床类型评分均有变化，临床治疗后得分有所提高。颜色变化与VAS呈相关变化。

Many kinds of diseases of oral mucosa are unknown etiology. Usually clinical pattern shows bullous, erosive, keratinize, ulcerative leasions, so leading to differential diagnosis is difficult.The purpose of in this study was to examine possible epithelial cell membrane changes in lichen planus affected by the use of the WGA and the color variation in various clinical types of the lichen planus (OLP).The results were obtained as follows.1) The binding of the epithelium to wheat germ agglutinin (WGA) was examined.In healthy oral mucousal epithelium, the binding pattern was obserbed regularly and strong but in OLP irregularly and weak in various clinical types especialy most decrease in lectin binding was observed in erosive lesion.2) We measured the color variation of the epithelium by CHROMA meter CS-100(MINOLTA). The color space (CIE 1976 L^<**>a^<**>b^<**>) and the color difference (CIE 1976 DELTAE^<**>ab) were examined.Compare to healthy mucousal epithelium with lichen planus, the color space L^<**> and b^<**> were minus quantity, a^<**> was plus quantity.The color differrence DELTAE^<**>ab was higher about 7th levels.3) We measured the pain caused by OLP quantativery, using a visual analogue scale (VAS). The score showed a change in various clinical types and better by clinical therapies. The color variation and VAS showed corelatively changes.