Roles of multifunctional adaptor proteins, GGAs, in membrane traffic

在本研究项目中，我们对GGAs及其相关的Tom 1家族蛋白进行了以下研究。(1)We发现ARF与GGA的GAT结构域的N-GAT亚结构域结合。(2)By通过X射线晶体学分析，我们揭示了与ARF复合的GGA 3-GAT结构域的三维结构。(3)We发现泛素与GGA和Tom 1家族蛋白的GAT结构域的C-GAT亚结构域结合。(4)By X射线晶体学，我们揭示了三维结构的GGA 3-GAT结构域和的Tom 1-GAT结构域的复合物与泛素。(5)We发现Tollip（Toll相互作用蛋白）与Tom 1家族蛋白的GAT结构域相互作用。(6)We Tollip将Tom 1家族蛋白和泛素化蛋白募集到核内体上。

In this research project, we revealed the followings on GGAs and its related Tom1 family proteins.(1)We found that ARFs bind to the N-GAT subdomain of the GAT domain of GGAs.(2)By X-ray crystallography, we revealed the three-dimensional structure of the GGA3-GAT domain in complex with ARF.(3)We found that ubiquitin bind to the C-GAT subdomain of the GAT domain of GGAs and Tom1 family proteins.(4)By X-ray crystallography, we revealed the three-dimensional structure of the GGA3-GAT domain and that of the Tom1-GAT domain in complex with ubiquitin.(5)We found that Tollip (Toll-interacting protein) interacts with the GAT domain of the Tom1 family proteins.(6)We revealed that Tollip recruits Tom1 family proteins and ubiquitinated proteins onto endosomes.