Development of clean up technology for chlorinated organic compounds by using mirobes having high degradability

1)采用末端限制性片段长度多态性（T-RFLP）分析了氯乙烯污染场地▲（大部分TCE污染物转移到顺式二氯乙烷）中出现的微生物种群。16 SrRNA基因克隆不是致病菌或机会性传染病致病菌。我们的结论是，该过程不会加速任何有害微生物的发展。2）氯乙烯还原酶基因vcrA的DNA序列的变化进行了分析，在两个三氯乙烯污染的网站。来自同一位点不同位点的所有vcrA克隆没有相同的DNA序列。结果表明，vcrA基因的某个位点随时间发生了变化。调查的两个地点显示出几乎相同的趋势。vcrA基因在7个清除位点中成为优势脱卤酶基因，表明VcrA是完全脱卤的关键酶。3）综上所述，具有vcrA基因的脱卤球菌菌株在TCE清除位点的完全脱卤过程中发挥着重要作用。该过程不会增加治疗期间产生的微生物的任何风险。

1) Microbial population appeared at chlorinated ethene contaminated sites▲(most of TCE contaminants were transferred to cis-DCE)were analyzed by Terminal Restriction Fragment Length Polymorphism (T-RFLP). Clones of 16S rRNA gene were not from disease-causing bacteria or opportunistic infectious disease causing bacteria. We concluded that the process does not accelerate development of any harmful microbes.2) Variations of DNA sequence of vinyl chloride reductase gene vcrA were analyzed at two TCE contaminated sites. All vcrA clones from different points at a site did not have the same DNA sequence. Slight changes in DNA sequences were found. This result suggest that a original vcrA gene has been changed with time at a site. Two sites surveyed showed almost the same trends. The gene of vcrA became a dominant dehalogenase gene among 7 cleaned up sites, showing that VcrA was a key enzyme for the complete dehalogenation.3) In summary, Dehalococcoides strains having vcrA gene was found to play an important role for the complete dehalogenation at TCE clean up sites. And the process did not increase any risks derived from microorganisms developed during the treatment period.