Constructive research for understanding the effects of amyloid β to neuronal function.

阿尔茨海默病(AD)是以淀粉样多肽(Aβ，Aβ)聚集为特征的疾病。本研究的目的是通过多电极阵列记录对神经细胞电活动的慢性监测，以及保护神经元免受A-β毒性的分子，来研究淀粉样β蛋白对胚胎大鼠海马区的影响。A-β作用于培养的胚胎大鼠海马神经元，对自发活动有抑制作用，作用于24小时后开始，72小时后放电消失。当黑种草油活性成分百里酚(TQ)与Aβ同时应用时，在用药后34h，百里酚可减轻Aβ的毒害作用。提示TQ对A-β毒性海马细胞具有潜在的保护作用。

Alzheimer's disease (AD) is characterized by the aggregation of amyloid β peptide (Aβ). The aim of this study is to investigate the effect of Amyloid Beta toxicity in embryonic rat hippocampus through chronic monitoring of neuronal cell electrical activity using multielectrode array (MEA) recordings, and the molecules protecting neurons from Aβ toxicity. Applying Aβ to cultured embryonic rat hippocampal neurons has an inhibitory effect on spontaneous activity started 24h after applying and the firing disappeared at 72h. When thymoquinone (TQ) active constituent of Nigella Sativa Oil and Aβ were simultaneously applied, thymoquinone reduces the deleterious effect of Aβ at 34h after applying. This results indicated that TQ have the potential protecting hippocampus cells from Aβ toxicity.