Elucidating signaling components under low potassium conditions

将拟南芥FOX（全长cDNA过表达基因）农杆菌文库转化到建立的AtHAK 5启动子：：荧光素酶拟南芥植物中。对这些AtHAK5启动子：：荧光素酶基因的FOX文库进行了筛选，获得了200多株在全营养条件下具有较高荧光素酶活性的候选转基因植株。其中，分析了15个株系以确定哪个基因过表达，并分析了一些候选株系响应营养缺乏的表达。获得了WRKY TF的过表达植株和敲除植株。过表达植株表现出对缺钾条件的耐受性增加。

Arabidopsis FOX (Full-length cDNA over-expressor genes) agrobacterium librarys were transformed into established AtHAK5 promoter::luciferase Arabidopsis plants. These AtHAK5 promoter::luciferase plants with FOX library have been screened and more than 200 candidate transgenic plants that have higher luciferase activity under full nutrient condition have been isolated. Among them, fifteen lines were analyzed to determine which gene is overexpressed, and some candidates were analyzed for their expression in response to nutrient deficiency. The overexpression plants and knockout plants of WRKY TF were obtained. The overexpression plants showed increased tolerance to potassium deficient condition.