Structural basis for the indirect aminoacyl-tRNA synthesis pathway from H. pyroli

我们一直专注于GatCAB的结构和功能分析，它在氨酰-tRNA合成的间接途径中具有重要作用。我们已经确定了古菌GatCAB的晶体结构在2。5 A决议。古细菌GatCAB和细菌GatCAB的结构比较表明，细菌GatCAB中的3_<10>螺旋对于识别tRNA α中的U1-A72对至关重要<Gln>，并且古细菌GatCAB中螺旋的缺失使得该酶能够识别具有G1-C72碱基对的aa-tRNA。

We have been focusing on the structural and functional analysis of GatCAB, which has an important role in an indirect pathway of aminoacyl-tRNA synthesis. We have determined the crystal structure of an archaeal GatCAB at 2. 5 A resolution. The structural comparison of the archaeal GatCAB and the bacterial GatCAB suggested that a 3_<10> helix in the bacterial GatCAB is crucial for recognition the U1-A72 pair in tRNA^<Gln>, and the absence of the helix in the archaeal GatCAB enables the enzyme recognize aa-tRNA with G1-C72 base-pairs.