Cell therapy for radiation-induced osteonecrosis with non-cultured adipose derived cells

颌骨骨坏死是头颈部癌症患者接受放射和/或化学治疗后的严重副作用。在某些情况下，它不仅限制了患者耐受癌症治疗的能力，而且对生活质量产生有害影响。然而，目前的临床策略不能有效地预防颌骨骨坏死的发生。因此，迫切需要开发有效的治疗方法。将从雄性C3 H小鼠分离的脂肪衍生细胞（ADC）通过尾静脉移植到照射后即刻的雌性小鼠。采用胶原酶消化和离心的简单方法从脂肪组织中分离脂肪来源细胞（ADC）。顺铂（7 mg/kg）16 Gy照射8周和12周后，取颌骨，进行组织学和免疫学分析，以明确ADC移植的效果。另一方面，直接植入新鲜分离的ADC与少量的BMP可以诱导异位骨形成。照射12周后，ADC移植小鼠骨组织中的细胞和血管闭塞较照射/未移植小鼠减少。

Jaw-Osteonecrosis is a serious side effect for patients with head and neck cancer who received radiation and/or chemo-therapy. It causes not only the limitation on the ability of patients to tolerate cancer therapies in some cases but also the harmful effects on the quality of life. However, current clinical strategies cannot efficiently prevent the occurrence of jaw-osteonecrosis. Therefore, effective therapy should be developed urgently. Adipose derived cells (ADC) isolated from male C3H mice were transplanted to the female mice just after the irradiation through the tail vein. Adipose derived cells (ADC) have been isolated from adipose tissue by the simple method with collagenase digestion and centrifugation. After 8 and 12 weeks of irradiation (16Gy) with cisplatin (7mg/kg), the jaw bone was harvested, and the histological and immunological analyses were performed to clarify the effects of ADC transplantation. On the other hand, the direct implantation of freshly isolated ADC with minimal amount of BMP could induce the ectopic bone formation. After 12 weeks of irradiation, theADC-transplanted mice reduced the obliteration of cell and blood-vessels in bone tissue compared with irradiated/non-transplanted mice.