Development of new Treatments for Osteoarthritis using Gene Transfer by Fullerene

开始了利用可溶性富勒烯治疗骨性关节炎的新方法的研究。在培养基中加入可溶性富勒烯。观察HIG-82细胞的反应。高密度区有抑制细胞增殖的趋势。但这并不重要。用酶联免疫吸附试验检测培养上清液中炎性细胞因子的含量。然而，没有观察到这种变化。这可能与培养液中的细胞因子有关。有必要考虑测量的方法。只有滑膜炎症细胞才能证实富勒烯的抗炎作用，目前还很难发现。未来需要对炎性滑膜细胞和正常滑膜细胞进行比较。此外，它有望加入机械应力作用于正常滑膜细胞，对其上，将继续研究富勒烯的抗炎作用。

Research for the purpose of a new treatment method using soluble fullerene to osteoarthritis was started. Soluble fullerene was added to the culture medium. Reaction of HIG-82 cells was observed. Trends suppression of cell proliferation was observed in the high density region. But it was not significant. Inflammatory cytokines in culture supernatants was measured by ELISA. However, the variation was not observed. These may be due to cytokine in the culture medium. It is necessary to consider the method of measurement. Only synovial inflammatory cells that confirm the anti-inflammatory effect of fullerene difficult have been found. Future comparison of inflammatory synovial cells and normal synovial cells is needed. In addition, it is expected to be added to the mechanical stress on normal synovial cells, on it, will continue to study the anti-inflammatory effect of the fullerene.