Identification of novel host factors responsible for the functional regulation of influenza virus ribonucleoprotein complexes

流感病毒的基因组在细胞核内复制。复制的病毒基因组形成核糖核蛋白(RNP)复合体。子代RNP复合体输出到细胞质并聚集在微管组织中心(MTOC)，然后沿着微管移动到细胞表面下的萌发位置。然而，RNP的复制和复制后过程的调控机制在很大程度上是未知的。在此，我们鉴定了YB-1是一种新的病毒RNP结合蛋白，它是一种细胞RNA结合蛋白。我们发现YB-1是将病毒RNP导向MTOC的转运蛋白。此外，我们还用生化方法检测了从高致病毒株或低致病毒株纯化的病毒聚合酶的酶活性。

The genome of influenza virus replicates in the nucleus. The replicated viral genome forms ribonucleoprotein (RNP) complexes. The progeny RNP complexes are exported to the cytoplasm and acculate in the microtubule-organizing center (MTOC) and then moves to the budding site beneath the cell surface along microtubules. However, the regulatory mechanism of replicational and post-replicational processes of the RNP are largely unknown. Here, we identified YB-1, which is a cellular RNA-binding protein, as a novel viral RNP binding protein. We found that YB-1 is a porter that leads viral RNP to MTOC. Further, we also examined enzymatic activities of viral polymerases purified from either high or low pathogenic strain using biochemical assays.