Mouse experimental myopia model and cell-type specific NGS profiling
Mouse experimental myopia model and cell-type specific NGS profiling
小鼠实验性近视模型和细胞类型特异性 NGS 分析
批准号：
24890100
负责人：
GOTOH NORIMOTO
金额：
$1.91万
依托单位：
依托单位国家：
日本
项目类别：
Grant-in-Aid for Research Activity Start-up
财政年份：
2012
资助国家：
日本
项目状态：
已结题
起止时间：
2012-08-31 至 2014-03-31
中文摘要
翻译
近视在日本人群中是一种常见的眼部状态，其患病率似乎超过40%。高度近视又称病理性近视，是东北亚地区视力威胁最大的疾病之一。有报道称增强近视的神经信号起源于视网膜内层，但目前尚不清楚哪一种细胞对近视信号的贡献最大。本研究利用流式细胞术（FACS）分离和富集小鼠神经节细胞，并对提取的RNA进行优化，利用下一代测序仪（NGS）进行RNA测序。该方法可应用于实验性近视小鼠，揭示近视的发病机制。
英文摘要
Myopia is a common ocular status in Japanese population, whose prevalence seems to be more than 40%. High myopia, also known as pathologic myopia, is one of the top vision threatening diseases in Northeastern Asian countries. It was reported that the neural signal enhancing myopia is originated at the inner layer of retina, but it is not clear which cell type has a largest contribution for the myopic signal. In this study, mouse ganglion cell was dissociated and enriched with flow-cytometry (FACS), and the extracted RNA was optimized for RNA Seq, performed with next generation sequencer (NGS). This approach can be applied for experimental myopic mice for revealing the pathomechanism of myopia.
期刊论文(2)
专著(0)
科研奖励(0)
会议论文
DOI：
10.1167/iovs.13-12825
发表时间：
2013-11-01
期刊：
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
影响因子：
4.4
作者：
[Oishi, Maho, Yamashiro, Kenji, Yoshimura, Nagahisa]
通讯作者：
Yoshimura, Nagahisa
Identification of the WNT7B gene provides the mechanism underlying myopia development
WNT7B基因的鉴定提供了近视发展的机制
WNT7B基因的鉴定提供了近视发展的机制
DOI：
--
发表时间：
期刊：
影响因子：
--
作者：
[Miyake M, Yamashiro K, Tabara Y, Suda K, Morooka S, Nakanishi H, Khor CC, Chen P, Nakata I, Akagi-Kurashige Y, Gotoh N, Tsujikawa A, the Nagahama Study Group, Meguro A, Kusuhara S, Polasek O, Hayward C, Wright AF, Campbell H, Richardson AJ, Schache M, Tak]
通讯作者：
Tak