Investigation of the function of nucleostemin in oral squamous cell carcinoma and develop the new therapeutic approoach

本研究旨在探讨核干素（NS）对口腔鳞状细胞癌（OSCC）患者恶性肿瘤的影响及其临床意义，并探索新的治疗方法。我们利用NS过表达或低表达的OSCC细胞，研究了NS对OSCC增殖和侵袭的影响。对OSCC患者进行NS免疫组织化学分析。NS的表达状态与OSCC细胞的恶性表型显著相关。这些恶性表型的改变与STAT3信号及其下游靶点的激活有关。免疫组织化学分析表明，高NS肿瘤表达水平与t分期和淋巴结状态显著相关。此外，Cox回归分析显示，NS状态是OSCC患者的重要进展因素。我们的研究结果表明，靶向NS可能为高度恶性OSCC提供了一种有希望的治疗方法。

The purpose of this study was to explore the impact of Nucleostemin (NS) on malignancy,its clinical significance in oral squamous cell carcinoma (OSCC) patients and develop new therapeutic approach. We investigated the effects of NS on the proliferation and invasion of OSCC using NS-overexpressing or -knockdown OSCC cells. An immunohistochemical analysis of NS was performed in OSCC patients. The expression status of NS significantly correlated to the malignant phenotypes of OSCC cells. The alterations of these malignant phenotypes were associated with the activation of STAT3 signaling and its downstream targets. An immunohistochemical analysis demonstrated that a high NS tumor expression level significantly correlated with an advanced T-stage and nodal status. Furthermore, a Cox regression analysis revealed that the NS status was a significant progression factor for OSCC patients.Our results suggest that targeting NS may provide a promising treatment for highly malignant OSCC.