The study of embryonic hemoglobin re-expression in adult mice.

哺乳动物造血发生两个不同的波，分别为原始波和终波；并表达不同类型的血红蛋白。然而，不存在这两种造血系统同时存在于成人的可能性。我们建立了用含二膦酸盐和苯肼处理的氮诱导危重性贫血的成年小鼠胚胎血红蛋白再表达的实验体系，并检测了在肝脏中同时表达红系标记和胚胎血红蛋白的细胞。我们还证实了成人和胚胎型血红蛋白亚基同时存在于外周血中。此外，还可以确认调节血红蛋白表达和转换的因子。这些结果支持理解血红蛋白在发育过程中的表达和转换机制。

Mammal hematopoiesis occurs two distinct waves, kwon as a primitive and definitive; and to express the hemoglobin of different types are known. However, there is no possibility that the hematopoietic system of these two simultaneous presence in the adult.We established an experimental system to re-express the embryonic hemoglobin in adult mice by inducing the critical anemia treated with nitrogen containing bisphosphonate and phenylhydrazine, and we detected the cell, expressing at the same time the erythroid marker and embryonic hemoglobin in the liver. We also confirmed that the hemoglobin subunites of adult and embryo types were simultaneously present in the peripheral blood. In addition, it was possible to confirm the factors, which regulates the expression and switching of hemoglobin. These results support that understanding the mechanism of hemoglobin expression and switching during development.