Glycoproteomic approach for identification of heart failure-related proteins as a novel biomarker

为了开发心力衰竭的治疗策略，迫切需要一种能够量化疾病程度的新型生物标志物。在本研究中，我们旨在利用糖蛋白组学方法鉴定一种新的生物标志物，该标志物可以作为现有标志物的有价值的补充，以更准确地诊断和预后心力衰竭。我们首先分析了高血压诱导的大鼠在心脏肥厚和心力衰竭期间左心室的糖基化谱，确定了反映疾病程度的糖基结构改变。我们的数据还表明，负责糖基化特征性改变的去糖基化酶的血浆酶活性将是肥厚的新生物标志物。此外，针对左心室和血浆的糖蛋白组学分析确定了40多种与心力衰竭相关的糖蛋白作为生物标志物候选。

To develop a therapeutic strategy for heart failure, a novel biomarker which enables to quantify the degree of the disease is highly required. In the present study, we aimed to identify a novel biomarker which could serve as a valuable supplement to the existing markers for more accurate diagnosis and prognosis of heart failure, using a glycoproteomic approach.We first analyzed a glycosylation profile in the left ventricle of hypertension-induced rats during cardiac hypertrophy and heart failure, identifying altered glycan structures reflecting the degree of the disease. Our data also indicated that plasma enzymatic activity of a deglycosylation enzyme responsible for the characteristically altered glycosylation would be a novel biomarker for hypertrophy. In addition, glycoproteomic analysis targeting the left ventricle and plasma identified over 40 glycoproteins related to heart failure as biomarker candidates.