HTLV-I associated lymph node and HTLV-I pathogenesis.

成人T细胞白血病/淋巴瘤(ATLL)是一种与人类T细胞白血病病毒I型(HTLVI)相关的人类恶性肿瘤，组织学通常呈多形性，但组织学并不统一。为了阐明组织学分型与预后的关系，我们对572例结节性T细胞淋巴瘤进行了重新分类。在所有病例中，使用Southern印迹分析检测HTLV-I前病毒DNA的克隆整合。572例患者分为3组：(A)克隆整合组[247例]，(B)HTLV-I前病毒DNA克隆整合组[66例]，(C)无克隆整合组(259例)。A组临床预后较B组和C组差。结论：A组为ATLL(HTLV-I相关性T细胞淋巴瘤)。(BR J Haematol 1998,101,703-11)可见克隆性染色体异常，但核型在数目和结构上都非常复杂。没有特定的ka…ATLL的核型异常较多。我们利用反向聚合酶链式反应和FISH技术对HTLY-I的整合位点进行了研究。证实了随机积分。18例中有15例HTLV-I整合染色体出现异常，特别是2例单纯性染色体异常，HILV-I整合到异常染色体，而不整合到正常染色体。因此，HTLY-I前病毒整合似乎与染色体异常有关。(癌症快报1998,132,203-12)最近发现的诱骗受体3(DcR3)与Fa.sL结合并抑制FasL诱导的细胞凋亡，被认为在肿瘤细胞的免疫逃逸系统中发挥作用。应用斑点杂交和原位杂交技术，对17例EB病毒(EBV)相关性淋巴瘤、7例成人T细胞白血病淋巴瘤(AILL)中DcR3基因的扩增和表达进行了分析。EBV相关的PAL和NKL在淋巴瘤细胞中显示DcR3的扩增和表达。AILL也有DcR3的表达和扩增。DcR3在反应性细胞中不表达。(癌症通讯，2000,160，89-97)bub基因是萌芽酵母有丝分裂检查点的组成部分。最近，在两个大肠肿瘤细胞系中发现了BUB的人类同源物，并检测到了hBUB1和hBUBR1的突变等位基因。我们通过检测hBUB1和hBUBR1的突变，以及Bax、胰岛素样生长因子和转化生长因子II型的最小复制错误，探讨了Checkpoint基因在ATLL染色体异常中的作用。10例ATLL中有4例检测到hBUB1或hBUBR1的显著突变/缺失。8例B细胞淋巴瘤中仅1例出现hBUBR1无义突变，ATLL和B细胞淋巴瘤均未发现MIN。(《癌症通讯》，2000,158,141-50)少

Adult T-cell leukemia/lymphoma (ATLL) is a human malignancy associated with human T-cell leukemia virus type I (HTLVI), and the histology usually indicates a pleomorphic type, but the histology is not uniform. To clarify the relation between the histological classification and prognosis, we reclassified 572 cases with nodal T-cell lymphoma. In all cases, the clonal integration of HTLV-I proviral DNA was examined, using a Southern blot analysis. 572 cases were classified into 3 groups ; (A) the cases with clonal integration [247 cases], (B) cases with AThA, and those without clonal integration of HTLV-I proviral DNA [66 cases}, (C) cases without A ILA [259 cases]. Group A clinically showed a poorer prognosis than groups B and C. In conclusion, group Awas determined to be ATLL (HTLV-I associated T-cell lymphoma). (Br J haematol 1998, 101, 703-11)ATLL demonstrate clonal chromosome abnormalities, but the karyotypes are very complicated in both number and structure. There are no specific ka … More ryotype abnormalities in ATLL. We investigated the integration site of HTLY-I, using inverse-PCR and FISH. The random integration was confirmed. In 15 of the 18 cases, the chromosomes with HTLV-I integration showed abnormalities, and, especially in 2 cases with simple chromosome abnonnalities, HILV-I integrated int the abnormal chromosome, but not into the normal chromosome. The HTLY-I proviral integration thus seems to, be associated with chromosome abnormalities. (Cancer Letters 1998, 132, 203-12)The recently identified decoy receptor 3 (DcR3) binds to Fa.sL and inhibits FasL-induced apoptosis, and is considered to play a role in the immune escape system of neoplastic cells. We analysed the amplification and expression of DcR3, using dot blot and in situ hybridization (ISH), in 45 cases, which included 17 cases with Epstein-Barr virus (EBV) associated lymphorna, 7 cases with adult T-cell leukemia lymphoma (AILL). EBV-associated PAL and NKL exhibited DcR3 amplification and expression in lymphoma cells. AILL also showed DcR3 expression and amplification. The DcR3 expression was not in the reactive cells. (Cancer Letter, 2000, 160, 89-97)The BUB gene is a component of the mitotic checkpoint in budding yeast. Recently, human homologues of the BUB were identified and mutant alleles of hBUB1 and hBUBR1 were detected in two colorectal tumor cell lines. We examined the role of checkpoint gene in the chromosomal abnormalities of ATLL by investigating mutations of hBUB1 and hBUBR1, and MIN of replication errors of BAX, insulin-like growth factor, and transforming growth factor type II. Significant mutations/deletion of hBUB1 or hBUBR1 were detected in 4 of 10 cases with ATLL. In contrast, only one of eight B cell lymphomas showed nonsense mutation of hBUBR1.None of the ATLL and B cell lymphomas showed MIN. (Cancer Letters, 2000, 158, 141-50) Less