Source and dynamics of dissolved and particulate proteins in the hydrosphere.

溶解性和颗粒性有机物的来源和动态是更好地理解全球碳循环的关键问题。本研究结果表明，水圈中溶解有机物和颗粒有机物的无生命池中存在特定的蛋白质分子。在海洋环境中，在各个海洋区域的溶解相中检测到的蛋白质不到30种。其中一种溶解蛋白被鉴定为存在于革兰氏阴性细菌铜绿假单胞菌外膜的一种称为孔蛋白的特殊蛋白质的同源物。通过免疫化学检测，另一种蛋白质也被鉴定为另一种类型的porine。在淡水环境中，我们还检测到几个溶解相的蛋白质分子，其中一个溶解蛋白被鉴定为来自革兰氏阴性细菌百日咳博德tella的另一种孔蛋白。通过分子质量在子午和垂直方向上的分布差异，确定了海洋中颗粒蛋白的两类特征：背景蛋白和特异性蛋白。特异性蛋白分布在有限的分子质量范围内，通过n端氨基酸序列分析，鉴定出其中一个特异性蛋白为存在于浮游植物叶绿体膜中的热休克蛋白70。这些蛋白质的发现提供了关于水圈和全球碳循环中无生命有机池的来源和动态的宝贵信息。

Source and dynamics of dissoloved and particulate organic matter is key question for better understanding of global carbon cycle. Results of this research indicated that there are specific protein molecules in the inanimate pools of dissolved and particulate organic matter in the hydrosphere. In marne environments, less than 30 proteins were detected in dissolved phase over the various oceanic areas. One of dissolved protein was identified as a homologue of a specific type of protein called porin, exists in the outer-membrane of the Gram-negative bacterium, Pseudomona aeruginosa. By means of immunochemical detection, one of the other protein also identified as another type of porine. In freshwater environment, we also detected several protein molecules in dissolved phase and one of the dissolved protein was identified as another type of porin from the Gram-negative bacterium, Bordetella pertussis.Two characteristic groups of particulate proteins in oceanic waters, background proteins and specific proteins, were identified from meridional and vertical differences in their molecular mass distributions. The specific proteins were distributed over a limited range of molecular masses and one of the specific protein was identified as the heat-shock protein 70, exists in the chloroplant membrane of phytoplankton, by the N-terminal amino acid sequence analysis. Discovery of occurrences of such proteins provide invaluable information on the source and dynamics of the inanimate organic pool in the hydorsphere and global carbon cycle as well.