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Studies of Earthworm Protease for Application of the Catalytic Function

Studies of Earthworm Protease for Application of the Catalytic Function
蚯蚓蛋白酶催化功能的应用研究
批准号:
10556023
负责人:
NAKAJIMA Nobuyoshi
金额:
$4.93万
依托单位国家:
日本
项目类别:
Grant-in-Aid for Scientific Research (B).
财政年份:
1998
资助国家:
日本
项目状态:
已结题
起止时间:
1998 至 2000

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中文摘要
翻译
本文对蚯蚓丝氨酸蛋白酶的催化功能进行了应用研究,得到了以下一些有用的结果.蛋白酶的纯化及性质研究.化学修饰的蛋白酶作为治疗酶.固定化酶生物反应器的构建。自溶物的分析与利用5.蛋白酶基因的克隆和表达.蛋白酶的结构、功能和稳定性的研究.研究蛋白酶的DNA和氨基酸序列。从酵母细胞中大规模生产重组蛋白酶。
英文摘要
Earthworm serine proteases were investigated for application of the catalytic function and the many useful results were obtained as bellows.1. Purification and characterization of the proteases.2. Chemical modification of the protease as a therapeutic enzyme.3. Construction of an immobilized enzyme bio-reactor.4. Analysis and utilization of the earthworm autolysate5. Gene cloning and expression of the proteases.6. Investigation of structure, function, and stability of the proteases.7. Investigation of DNA and amino acid sequences of the proteases.8. Large-scale production of the recombinant protease from Yeast cells.
期刊论文(54)
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会议论文
N.Nakajima, et.al.,: "Degradation of Pectic Substances by Two Pectate Cyases from a Human Intestinal Bacterium, Clostridium butyticum-beijerinckii."J.Biosci.Bioeng.,. 88(3). 331-333 (1999)
N.Nakajima 等人:“人肠道细菌拜氏梭菌 (Clostridium butyticum-beijerinckii) 中的两种果胶酸氰化酶对果胶物质的降解。”J.Biosci.Bioeng.,。
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通讯作者:
N.Nakajima, et.al.,: "Further characterization of Earthworm Serine Protease : Cleavage Specificity Against Peptide Substrates and on Autolysis."Biosci.Biotechnol.Biochem.,. 63(11). 2031-2033 (1999)
N.Nakajima 等人:“蚯蚓丝氨酸蛋白酶的进一步表征:针对肽底物和自溶的切割特异性。”Biosci.Biotechnol.Biochem.,。
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K.Ishihara,N.Nakajima, et.al.: "A chemoenzymatic Synthesis of Aromatic Carboxylic acid vinyl esters."J.Mol.Catal.B:Enzymatic,. 7. 307-310 (1999)
K.Ishihara,N.Nakajima, et.al.:“芳香族羧酸乙烯基酯的化学酶合成。”J.Mol.Catal.B:Enzymatic,。
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N.Nakajima,K.Ishihara,Y.Matsuura.,et al.: "Degradation of Pectic Substances in Human Intestine:Contribution and Action Pattern of Pectate Lyase from an Intestinal Bacteriam" Biosci・Biotech.Biochem.63(in press). (1999)
N. Nakajima、K. Ishihara、Y. Matsuura. 等人:“人类肠道中果胶物质的降解:肠道细菌果胶酸裂解酶的贡献和作用模式”Biosci・Biotech.Biochem.63(印刷中)。 (1999)
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共 23 条
    Recycling recovery of biomass and refinaly of the several useful compounds using the enzymatic coupling reaction system from earthworm and the enzymes from it's intesitinarl microorganisums.
    • 批准号:
      20580101
    • 项目类别:
      Grant-in-Aid for Scientific Research (C)
    • 资助金额:
      $3.0万
    • 财政年份:
      2008
    • 负责人:
      NAKAJIMA Nobuyoshi
    • 依托单位:
    Improvement of uptake of endocrine disruptors by plants
    Enzymatic stabilization and functionarization of polyphenol glucosides such as plant pigments for further utilization of their bio-functions.
    • 批准号:
      15580083
    • 项目类别:
      Grant-in-Aid for Scientific Research (C)
    • 资助金额:
      $2.18万
    • 财政年份:
      2003
    • 负责人:
      NAKAJIMA Nobuyoshi
    • 依托单位:
    海外基金