Establishment and use of recombinant inbred rat strains

LEXF/FXLE是由LE/STM和F344/STM杂交而成的重组近交系。开发它们的最初目的是定位丙基亚硝脲诱导的T淋巴瘤的易感基因。LEXF RI株定位于RN07，其中D7Mit4的显着性最高。为了进一步增加作为分析复杂遗传性状的遗传工具的有效性，我们扩展了独立的RI菌株的数量，并扩展了菌株分布模式(SDP)表。目前，我们有26个独立的RI菌株(11个LEXF和15个FXLE品系)和13个LEXF RI品系的亚系。利用LEXF/FXLE RI大鼠，对高脂饲料引起的高脂血症、链脲佐菌素(STZ)引起的高血糖和己烯雌酚(DBS)引起的睾丸重量变化三个复杂的遗传性状进行了分析。用MapManager QTL(Ver.X)进行了TRAU连锁(QTL)分析。通过对这些RI大鼠品系的QTL分析，发现了几个与饮食或药物诱导的表型有关的新基因座。LEXF/FXLE RI大鼠可用于复杂疾病的遗传解剖和药物敏感性研究。这些RI菌株目前保存在动物繁殖研究所(茨城)和理研生物资源中心理研筑波研究所。

The LEXF/FXLE, a recombinant inbred (RI) strain of rat, are derived from an intercross between LE/Stm and F344/Stm. The initial purpose in developing them was to map the susceptibility gene for T-lymphomas induced by propylnitrosourea. With LEXF RI strains, it was mappped on RN07, with highest significance at D7Mit4. To further increase the usefulness as a genetic tool for analyze complex genetic traits, we extended the numbers of independent RI strains, and expanded the strain distribution pattern (SDP) table.At present we have 26 independed RI strains (11 LEXF and 15 FXLE strains) and 13 sub-trains of LEXF RI strains. Using the LEXF/FXLE RI rat, we attempted to analyze threecomplicated genetic traits : Hyperlipidemia indeced by high fat diet, hyperglicemiaindeced by streptozotocin (STZ), and testis weight change induced by diethlstilbestrol (DBS). Quantitative traU linkage (QTL) analysis was done with Mapmanager QTL(Ver.X). Several new loci responsible for diet or drug-induced phenotype were identified by QTL analysis with these RI rat strais. The LEXF/FXLE RI rats are useful for genetic dissection of complex diseases and drug susceptibility. These RI strains were maintained at present in the Institute for Animal Reproduction (Ibaraki) and in the RIKEN BioResouce Center RIKEN Tsukuba Institute.