Bioartificial Endocrine Pancreas (Bio-AEP) for Treatment of Diabetes by using of a tissue-engineering technique and regenerative medicine.

我们描述了一种用于生物人工内分泌胰腺（Bio-AEP）的扩散室。Bio-AEP装置中的胰腺内分泌细胞通过人工屏障与宿主的免疫系统隔离，而营养物质、电解质、氧气和生物活性分泌产物通过他的屏障进行交换。(1)成年猪胰腺内分泌细胞的制备与纯化。(2)胰腺内分泌细胞单层培养的建立。(3)猪胰腺细胞的分泌特性和形态学特征。(4)长期培养对猪胰腺内分泌细胞抗原表达的影响。(5)将Bio-AEP异种植入全胰腺切除犬和自发性糖尿病猴。(6)研究PERV感染。(7)新生猪胰腺干细胞向胰岛素分泌细胞的分化。

We described a diffusion chamber for a bioartificial endocrine pancreas (Bio-AEP). Pancreatic endocrine cells in the Bio-AEP device were isolated from the immune system of the host by an artificial barrier while nutrients, electrolytes, oxygen and bioactive secretory products were exchanged across his barrier.(1) Preparation and purification of adult porcine pancreatic endocrine cells forth donor.(2) Establishment of monolayer culture of the pancreatic endocrine cells.(3) Characterization of secretory and morphologic properties of the porcine pancreatic cells.(4) Effect of long-term culture on the antigen expression in the porcine pancreatic endocrine cells.(5) Xenoimplantation of the Bio-AEPs to total pancreatectomized dogs and spontaneous diabetic monkeys.(6) Studies of PERV-infections.(7) Differentiation of the neonatal porcine pancreatic stem cell into insulin producing cells.