Study on biological mechanism, specially, information transmittance by malting use of high resolution spectroscopic method

本项目在金泽大学新建了一台喷嘴-射流傅立叶变换微波光谱仪，并对神奈川工业大学现有的FTMW进行了改造。利用这两台FTMW光谱仪，以及美国国家标准与技术研究院（NIST）的仪器。通过协同工作，研究肽分子和其他分子物种的纯旋转光谱，获得分子内相互作用的信息，有助于理解生物信息传递的机制。本项目研究的分子种类有甲基膦酸二甲酯、N-甲基丙酰胺、N-甲基甲酰胺、N-甲基乙酰胺、1,1-二甲基硅环丁烷、N-甲基苯胺、N，N-二甲基乙酰胺、ar -二甲醚配合物、ne -二甲醚配合物等。例如，对于N，N-二甲基乙酰胺，通过全面旋转和内部旋转之间的科里奥利相互作用，获得了与三个ch_3 -内旋转的传递率相关的跃迁概率；对于1,1-二甲基硅环丁烷分子，确定了ch_3 -内旋转和环折叠运动之间相互作用的旋转常数的贡献。涉及这些量的信息是有用的，它们被认为与形态学相关分子的信息透射率密切相关。

In the present project, a nozzle-jet Fourier transform microwave (ETTMW) spectrometer was newly constructed in Kanazawa University, and the FTMW existing already in Kanagawa Institute of Technology was revised. Using these two FTMW spectrometers, and also using the instrument in National Institute of Standards and Technology (NIST) of U.S,A. through collaborative work, pure rotational spectra of peptide molecules and other molecular species involving large-amplitude motions were investigated to obtain information on intra-moleculaar interactions useful for understanding mechanism of biological information transmittance. Molecular species studied in the present project are dimethyl methylphosphonate, N-methylpropionamide, N-methylformamide, N-methylacetamide, 1,1-dimethylsilacyclobutane, N-methylaniline, N,N-dimethylacetamide, Ar-dimethyl ether complex, Ne-dimethyl ether complex etc. For examples, a transition probability relating to transmittanee of the three CH_3-internal rotations occurring through Coriolis interaction between overall-rotation and internal-rotation was obtained for the N,N-dimethylacetamide, and a contribution to a rotational constant of the interaction between CH_3-internal rotation and ring-puckering motion was determined for the 1,1-dimethylsilacyclobutane molecule. Information involved in these quantities is useful, which are considered to be strongly related to information transmittance of fiologically related molecules.