High-resolution proteomic analysis of native sodium-phosphate transporters SLC34A1 and SLC34A3 and their role in kidney disease (P09)

P9将通过分析天然转运体NaPi-IIa （SLC34A1）和NaPi-IIc （SLC34A3）在哺乳动物肾脏中的亚基组成、运作和细胞生理来研究它们的作用。编码这些蛋白的基因的罕见突变导致磷酸盐代谢紊乱和肾结石疾病，GWAS显示SLC34A1与血清磷酸盐水平和肾脏滤过过程的改变有关。P9将使用多学科、互动的方法，包括定量质谱和先前开发的生化和蛋白质组学技术，包括复杂组分析，以及功能分析、荧光和电子显微镜来阐明这些肾脏疾病靶点的调控、功能和相互作用伙伴。

P9 will study the role of native transporters NaPi-IIa (SLC34A1) and NaPi-IIc (SLC34A3) by analyzing their subunit composition, operation and cell physiology in the mammalian kidney. Rare mutations in the genes encoding these proteins cause disorders of phosphate metabolism and kidney stone disease, and GWAS showed that SLC34A1 is associated with alterations in serum phosphate levels and the filtration process in kidney. P9 will use a multidisciplinary, interactive approach comprising quantitative mass spectrometry and previously developed biochemical and proteomic techniques including complexome profiling, together with functional analyses as well as fluorescence and electron microscopy to elucidate the regulation, function and interaction partners of these kidney disease targets.