Ergogenic effects of β_2-agonist, clenbuterol and glucocorticoid in rats

研究了/β_2激动剂盐酸克仑特罗（CLE: 0.01 ~ 1.0 mg/kg BW）对大鼠血浆葡萄糖、甘油三酯、总胆固醇、胰岛素和环AMP浓度及血浆体积比的影响（1- 1实验）。CLE （1.0 mg/kg体重/天，连用10 d）、全身悬浮（WBS，连用10 d）和固定化（IMM，连用10 d）对大鼠白细胞数量的影响（第2实验）。1- 1实验：1)CLE显著升高血浆葡萄糖、甘油三酯、总胆固醇和胰岛素浓度，且呈剂量依赖性。2)CLE显著提高血浆体积比（=血浆体积与总血容量之比）呈剂量依赖性，且在给药后10小时以上血浆体积比的升高保持不变，提示CLE对细胞外液容量有增加作用。3)给药后血浆环AMP浓度无明显变化。这些结果表明，CLE增加了血浆葡萄糖、甘油三酯、总胆固醇、胰岛素浓度和细胞外液体积（=血浆体积比），但不改变血浆环AMP浓度。第2个实验：1)IMM组、WBS组总白细胞数量显著高于对照组（CON）。然而，在给药中没有观察到白细胞总数的影响。2) IMM、WBS和cle给药组中性粒细胞和单核细胞数量显著高于CON组。3)淋巴细胞数量不随IMM和WBS变化。然而，与CON组相比，给予cle显著降低淋巴细胞数量约为0.56倍。4)与对照组相比，IMM组和WBS组的嗜碱性粒细胞数量显著增加。然而，在给药中没有观察到嗜碱性粒细胞数量的显著影响。5) IMM和WBS中嗜酸性粒细胞数量显著高于CON组。相反，cle给药组的嗜酸性粒细胞数量约为CON组的0.53倍。6) 3组胸腺重量均较CON组显著降低0.65 ~ 0.83倍。三组患者的肾上腺质量均高于对照组。综上所述，IMM、WBS和cle均显著增加了中性粒细胞和单核细胞的数量。虽然IMM和WBS增加了白细胞总数，但WBS比IMM更明显地增加了单核细胞、淋巴细胞和嗜碱性粒细胞的数量。然而，给药可减少淋巴细胞和嗜酸性粒细胞的数量。少

The ergogenic effects of/β_2-agonist, clenbuterol (CLE : 0.01-1.0 mg/kg BW) on plasma glucose, triglyceride, total cholesterol, insulin and cyclic AMP concentrations, and plasma volume ratio were studied in rats (1-st Experiment). Effects of CLE (1.0 mg/kg BW/day for 10 days), whole body suspension (WBS for 10 days) and immobilization (IMM for 10 days) on the number of white blood cells in rats (2-nd Experiment).1-st Experiment :1)CLE increased significantly the plasma glucose, triglyceride, total cholesterol and insulin concentrations in the dose-dependent manner. 2)CLE increased significantly the plasma volume ratio(=ratio of plasma volume to total blood volume) in the dose-dependent manner and the increase of plasma volume ratio was maintained over 10 hours after the admistration, suggesting the increasing action of CLE to extracellular fluid volume. 3)No significant changes of plasma cyclic AMP concentration were observed in CLE admistration. These results indicate that CLE increas … More es dose-dependently plasma glucose, triglyceride, total cholesterol, insulin concentrations, and extracellullar fluid volume (=plasma volume ratio) without changing plasma cyclic AMP concentrations.2-nd Experiment :1)Numbers of total white blood cells in the IMM, WBS groups were significantly higher than those in the control (CON) groups. No effects of numbers of total white blood cells, however, were observed in CLE-administration. 2)Numbers of neutrophil and monocyte in the IMM, WBS and CLE-administration groups were significantly higher than those in the CON groups. 3)Numbers of lymphocytes did not change with the IMM and WBS. CLE-administration, however, decreased significantly the numbers of lymphocytes to approximately 0.56 times, as compared with the CON group. 4)The IMM and WBS increased signicantly the numbers of basophil than in the CON groups. No significant effects of the numbers of basophil, however, were observed in CLE-administration. 5)The IMM and WBS increased significantly the numbers of eosinophil than in the CON groups. On the contrary, the numbers of eosinophil in CLE-administration group were about 0.53 times significantly lower than those in the CON group. 6)Thymus weights in three groupswere 0.65-0.83 times significantly lower than in the CON groups. Adrenal weights in three groups were also relatively higher than those in the CON groups.In conclusion, the IMM, WBS and CLE-administration increased markedly the numbers of neutrophils and monocytes. Although the IMM and WBS increased total white blood cells, the WBS increased more markedly the numbers of monocytes, lymphocytes and basophils than in the IMM. CLE-administration, however, decreased the numbers of lymphocytes and eosinophils. Less