Analysis of the hydroperoxides component in oxidi zed LDL

目的内皮细胞氧化脂蛋白在动脉硬化的发生中起重要作用。近年来，血浆中氧化脂蛋白的存在已被证实。然而，氧化型脂蛋白在血浆中的化学性质和代谢调控机制尚不清楚。本研究的目的是对人血清中氧化低密度脂蛋白（LDL）中的脂质成分（过氧化氢）进行详细的定性和定量分析，并与几种脂质相关疾病进行相关性研究。这些氢过氧化物的结构通过色谱分离为各非对映异构体后的光谱数据确认。然后通过上述反应合成了三酰基甘油的氢过氧化物，并通过光敏氧化合成了三酰基甘油。利用这些合成的过氧化氢，建立了高效液相色谱（荧光检测和荧光检测）分析方法，结果表明，LDL组分中的过氧化氢含量高于HDL组分，说明LDL组分中大部分存在脂质过氧化氢，即过氧化氢。对胆固醇转移蛋白（CETP）缺乏症和CD_（36）缺乏症等血脂异常患者血清中LDL和HDL组分中过氧化氢的分析，目前尚在研究中，其结果应在其他文献中发表。本研究的目的之一是建立一种简便的血清氢过氧化物测定方法，例如EIA方法。EIA方法的开发研究目前也在调查中，以使用胆固醇亚油酸氢过氧化物作为半抗原。

PurposeIt is known that oxidized lipoprotein in the endodermis is important for the generation of the arteriosclerosis. Recently, the existence of the oxidized lipoprotein in blood plasma has been confirmed. However, chemical property and metabolic regulation mechanism of the oxidized lipoprotein in blood plasma arenot sufficiently known. The purpose of the present study carries out details qualitative analysis and quantitative analysis on lipid component (hydroperoxides) of the oxidized LDL in human serum in connection with several lipid associated disorders.Results and ExperimentalsIn order to develop the analytical methods of hydroperoxides, the hydroperoxides of cholesterol esters (oleic, linoleic, linolenic acid esters) were first synthesized by the photo-sensitized oxidation. The structures of these hydroperoxides were confirmed by their spectral data after chromatographic separation into each diastereoisomeric isomer. Then hydroperoxides of triacyl glycerol are also synthesized by the above reactions, and triacy glycerols were synthesized by the photo-sensitized oxidation. Using these synthesized hydroperoxides, the analytical methods by high-performance liquid chromatography (fluorescent detection and chemiluminescent detection) were established and the present methods were peroxides in LDL fraction are higher than that of HDL fraction, showing that lipid hydroperoxides are existed in mostly LDL Fraction, i. e, oxidized LDL.The analysis of the hydroperoxides in LDL and HDL fraction of the serum from Lipid disorders, such as cholesterol transfer proten (CETP) deficiency and CD36 deficiency, are now under investigation and the results should be apperred elsewhere. One of the aime of this research is the development of convenient assay of hydroperoxide in serum., such as EIA method. The research for development of the EIA method is now also under investigation to use cholesteryl linoleate hydroperoxides as hapten.